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Literature DB >> 21075891

Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis.

M C Portillo¹, D Villahermosa, A Corzo, J M Gonzalez.

Abstract

Complex microbial communities exhibit a large diversity, hampering differentiation by DNA fingerprinting. Herein, differential display-denaturing gradient gel electrophoresis is proposed. By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison.

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Year: 2010 PMID： 21075891 PMCID： PMC3019698 DOI： 10.1128/AEM.01316-10

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

27 in total

1. Numerical analysis of grassland bacterial community structure under different land management regimens by using 16S ribosomal DNA sequence data and denaturing gradient gel electrophoresis banding patterns.

Authors: A E McCaig; L A Glover; J I Prosser
Journal: Appl Environ Microbiol Date: 2001-10 Impact factor: 4.792

2. DGGE analysis of 16S rDNA of ammonia-oxidizing bacteria in chemical-biological flocculation and chemical coagulation systems.

Authors: Siqing Xia; Yan Shi; Yigang Fu; Xingmao Ma
Journal: Appl Microbiol Biotechnol Date: 2005-10-20 Impact factor: 4.813

3. Effect of sludge age on the bacterial diversity of bench scale sequencing batch reactors.

Authors: Alper Tunga Akarsubasi; Ozge Eyice; Ian Miskin; Ian M Head; Thomas P Curtis
Journal: Environ Sci Technol Date: 2009-04-15 Impact factor: 9.028

4. Differential microbial communities in hot spring mats from Western Thailand.

Authors: M C Portillo; V Sririn; W Kanoksilapatham; J M Gonzalez
Journal: Extremophiles Date: 2008-12-25 Impact factor: 2.395

5. Composition and diversity of ammonia-oxidising bacterial communities in wastewater treatment reactors of different design treating identical wastewater.

Authors: Arlene K Rowan; Jason R Snape; David Fearnside; Michael R Barer; Thomas P Curtis; Ian M Head
Journal: FEMS Microbiol Ecol Date: 2003-03-01 Impact factor: 4.194

6. Analysis of the dynamics of bacterial communities in the rhizosphere of the chrysanthemum via denaturing gradient gel electrophoresis and substrate utilization patterns

Authors:
Journal: Appl Environ Microbiol Date: 1998-12 Impact factor: 4.792

Review 7. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.

Authors: G Muyzer; K Smalla
Journal: Antonie Van Leeuwenhoek Date: 1998-01 Impact factor: 2.271

8. Nitrate promotes biological oxidation of sulfide in wastewaters: experiment at plant-scale.

Authors: Juan García de Lomas; Alfonso Corzo; Juan M Gonzalez; Jose A Andrades; Emilio Iglesias; María José Montero
Journal: Biotechnol Bioeng Date: 2006-03-05 Impact factor: 4.530

9. Differential display by PCR: novel findings and applications.

Authors: M J Guimarães; F Lee; A Zlotnik; T McClanahan
Journal: Nucleic Acids Res Date: 1995-05-25 Impact factor: 16.971

10. Estimation of the abundance of an uncultured soil bacterial strain by a competitive quantitative PCR method.

Authors: S Y Lee; J Bollinger; D Bezdicek; A Ogram
Journal: Appl Environ Microbiol Date: 1996-10 Impact factor: 4.792

5 in total

Review 1. Nutrient scavenging activity and antagonistic factors of non-photobiont lichen-associated bacteria: a review.

Authors: M Auður Sigurbjörnsdóttir; Ólafur S Andrésson; Oddur Vilhelmsson
Journal: World J Microbiol Biotechnol Date: 2016-03-01 Impact factor: 3.312

2. Detection of Bacillus and Stenotrophomonas species growing in an organic acid and endocrine-disrupting chemical-rich environment of distillery spent wash and its phytotoxicity.

Authors: Ram Chandra; Vineet Kumar
Journal: Environ Monit Assess Date: 2016-12-20 Impact factor: 2.513