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Literature DB >> 2107532

Internal eliminated sequences are removed prior to chromosome fragmentation during development in Euplotes crassus.

Abstract

The hypotrichous ciliated protozoa undergo a massive genome rearrangement process after their sexual cycle. One frequent type of rearrangement is the removal of DNA sequences (internal eliminated sequences; IESs) from internal regions of DNA molecules. In this study, we characterized the removal of IESs in Euplotes crassus. Southern hybridization analyses combined with cytological observations indicated that IES removal is an early event in macronuclear development, occurring during the polytene chromosome stage and prior to the chromosome fragmentation process. The results are consistent with IES removal occurring via an intramolecular DNA breakage and rejoining process.

Entities: Chemical Species

Mesh：

Substances：
DNA

Year: 1990 PMID： 2107532 PMCID： PMC330336 DOI： 10.1093/nar/18.4.845

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

29 in total

1. Micronuclear genome organization in Euplotes crassus: a transposonlike element is removed during macronuclear development.

Authors: S E Baird; G M Fino; S L Tausta; L A Klobutcher
Journal: Mol Cell Biol Date: 1989-09 Impact factor: 4.272

2. Detection of circular forms of eliminated DNA during macronuclear development in E. crassus.

Authors: S L Tausta; L A Klobutcher
Journal: Cell Date: 1989-12-22 Impact factor: 41.582

3. Chromosomal rearrangement generating a composite gene for a developmental transcription factor.

Authors: P Stragier; B Kunkel; L Kroos; R Losick
Journal: Science Date: 1989-01-27 Impact factor: 47.728

4. Precise excision of telomere-bearing transposons during Oxytricha fallax macronuclear development.

Authors: D J Hunter; K Williams; S Cartinhour; G Herrick
Journal: Genes Dev Date: 1989-12 Impact factor: 11.361

5. Reordering of nine exons is necessary to form a functional actin gene in Oxytricha nova.

Authors: A F Greslin; D M Prescott; Y Oka; S H Loukin; J C Chappell
Journal: Proc Natl Acad Sci U S A Date: 1989-08 Impact factor: 11.205

6. Developmentally coordinated en masse excision of a highly repetitive element in E. crassus.

Authors: C L Jahn; M F Krikau; S Shyman
Journal: Cell Date: 1989-12-22 Impact factor: 41.582

7. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.

Authors: P J Farabaugh; U Schmeissner; M Hofer; J H Miller
Journal: J Mol Biol Date: 1978-12-25 Impact factor: 5.469

8. Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday.

Authors: G Streisinger; Y Okada; J Emrich; J Newton; A Tsugita; E Terzaghi; M Inouye
Journal: Cold Spring Harb Symp Quant Biol Date: 1966

9. Nuclear roles in the postconjugant development of the ciliated protozoan Euplotes aediculatus. 1. Evidence for sequential roles of the differentiating macronucleus in exconjugant development.

Authors: J A Kloetzel
Journal: Dev Biol Date: 1981-04-15 Impact factor: 3.582

10. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus.

Authors: L A Klobutcher; M T Swanton; P Donini; D M Prescott
Journal: Proc Natl Acad Sci U S A Date: 1981-05 Impact factor: 11.205

18 in total

8. Sequential excision of internal eliminated DNA sequences in the differentiating macronucleus of the hypotrichous ciliate Stylonychia lemnae.

Authors: J Wen; C Maercker; H J Lipps
Journal: Nucleic Acids Res Date: 1996-11-15 Impact factor: 16.971

9. Volatility of internal eliminated segments in germ line genes of hypotrichous ciliates.

Authors: M L DuBois; D M Prescott
Journal: Mol Cell Biol Date: 1997-01 Impact factor: 4.272

10. Timing of differential amplification of macronucleus-destined sequences during macronuclear development in the hypotrichous ciliate Euplotes crassus.

Authors: T Dönhoff; A Klein
Journal: Chromosoma Date: 1996-09 Impact factor: 4.316

Internal eliminated sequences are removed prior to chromosome fragmentation during development in Euplotes crassus.

1. Micronuclear genome organization in Euplotes crassus: a transposonlike element is removed during macronuclear development.

2. Detection of circular forms of eliminated DNA during macronuclear development in E. crassus.

3. Chromosomal rearrangement generating a composite gene for a developmental transcription factor.

4. Precise excision of telomere-bearing transposons during Oxytricha fallax macronuclear development.

5. Reordering of nine exons is necessary to form a functional actin gene in Oxytricha nova.

6. Developmentally coordinated en masse excision of a highly repetitive element in E. crassus.

7. Genetic studies of the lac repressor. VII. On the molecular nature of spontaneous hotspots in the lacI gene of Escherichia coli.

8. Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday.

9. Nuclear roles in the postconjugant development of the ciliated protozoan Euplotes aediculatus. 1. Evidence for sequential roles of the differentiating macronucleus in exconjugant development.

10. All gene-sized DNA molecules in four species of hypotrichs have the same terminal sequence and an unusual 3' terminus.

1. Timing of developmentally programmed excision and circularization of Paramecium internal eliminated sequences.

2. A unique pattern of intrastrand anomalies in base composition of the DNA in hypotrichs.

3. De novo telomere addition to spacer sequences prior to their developmental degradation in Euplotes crassus.

4. High fidelity developmental excision of Tec1 transposons and internal eliminated sequences in Euplotes crassus.

5. Conserved DNA sequences adjacent to chromosome fragmentation and telomere addition sites in Euplotes crassus.

6. Differential replication and DNA elimination in the polytene chromosomes of Euplotes crassus.

7. A nascent micronuclear pseudogene in the ciliate Euplotes crassus.

8. Sequential excision of internal eliminated DNA sequences in the differentiating macronucleus of the hypotrichous ciliate Stylonychia lemnae.

9. Volatility of internal eliminated segments in germ line genes of hypotrichous ciliates.

10. Timing of differential amplification of macronucleus-destined sequences during macronuclear development in the hypotrichous ciliate Euplotes crassus.