Literature DB >> 2107125

Development of a beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis.

P Haima1, D van Sinderen, H Schotting, S Bron, G Venema.   

Abstract

A versatile beta-galactosidase alpha-complementation system for Bacillus subtilis was developed, which can be used for molecular cloning in this Gram+ organism. The cloning system, which is based on the highly efficient host-vector system 6GM-pHP13, offers several advantages over previously described systems: (1) convenient direct selection of recombinants; (2) the cloning of large heterologous DNA fragments with high efficiency; and (3) the availability of six unique target sites: SphI, NdeI, NheI, BamHI, SmaI and EcoRI.

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Year:  1990        PMID： 2107125     DOI： 10.1016/0378-1119(90)90114-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  19 in total

1.  Characterization of a novel partition system encoded by the delta and omega genes from the streptococcal plasmid pSM19035.

Authors:  Michal Dmowski; Izabela Sitkiewicz; Piotr Ceglowski
Journal:  J Bacteriol       Date:  2006-06       Impact factor: 3.490

2.  Hpt, a bacterial homolog of the microsomal glucose- 6-phosphate translocase, mediates rapid intracellular proliferation in Listeria.

Authors:  Isabel Chico-Calero; Mónica Suárez; Bruno González-Zorn; Mariela Scortti; Jörg Slaghuis; Werner Goebel; José A Vázquez-Boland
Journal:  Proc Natl Acad Sci U S A       Date:  2001-12-26       Impact factor: 11.205

3.  Evidence that the Bacillus subtilis pyrimidine regulatory protein PyrR acts by binding to pyr mRNA at three sites in vivo.

Authors:  Y Lu; R L Switzer
Journal:  J Bacteriol       Date:  1996-10       Impact factor: 3.490

4.  Polyhydroxyalkanoate inclusion body-associated proteins and coding region in Bacillus megaterium.

Authors:  G J McCool; M C Cannon
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

5.  Identification of a novel gene of pyrimidine nucleotide biosynthesis, pyrDII, that is required for dihydroorotate dehydrogenase activity in Bacillus subtilis.

Authors:  A E Kahler; R L Switzer
Journal:  J Bacteriol       Date:  1996-08       Impact factor: 3.490

6.  Molecular analysis of the tagF gene, encoding CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase of Staphylococcus epidermidis ATCC 14990.

Authors:  S N Fitzgerald; T J Foster
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

7.  PhaC and PhaR are required for polyhydroxyalkanoic acid synthase activity in Bacillus megaterium.

Authors:  G J McCool; M C Cannon
Journal:  J Bacteriol       Date:  2001-07       Impact factor: 3.490

8.  The pyrimidine biosynthesis operon of the thermophile Bacillus caldolyticus includes genes for uracil phosphoribosyltransferase and uracil permease.

Authors:  S Y Ghim; J Neuhard
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

9.  RNA structure and heterologous recombination in the double-stranded RNA bacteriophage phi 6.

Authors:  S Onodera; X Qiao; P Gottlieb; J Strassman; M Frilander; L Mindich
Journal:  J Virol       Date:  1993-08       Impact factor: 5.103

10.  A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product.

Authors:  B van den Burg; H G Enequist; M E van der Haar; V G Eijsink; B K Stulp; G Venema
Journal:  J Bacteriol       Date:  1991-07       Impact factor: 3.490
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