PURPOSE: The exploration of immune mechanisms of the tumour-inhibitory effect of exposures to low-level fractions of X-rays. MATERIALS AND METHODS: BALB/c mice were exposed to whole-body daily irradiations with 0.01, 0.02, or 0.1 Gy X-rays per day for 5 days/week for two weeks. Then, mice were intravenously injected with L1 tumour cells, killed 14 days later, and neoplastic colonies were counted in the lungs. Natural killer (NK) cell-enriched splenocytes and activated peritoneal macrophages (Mϕ) were collected and cytotoxic activities of these cells against susceptible tumour targets were assayed. Concanamycin A (CMA) and antibody against the ligand for the Fas receptor (FasL) were used to inhibit the NK cell-mediated cytotoxicity. Production of nitric oxide (NO) was quantified using the Griess reagent. Secretion of interferon-γ (IFN-γ), interleukin-1β (IL-1β), interleukin-12 (IL-12), and tumour necrosis factor-α (TNF-α) was measured using the enzyme-linked immunosorbent assays. RESULTS: All the exposures to X-rays significantly reduced the number of the induced tumour colonies and enhanced cytotoxic properties of the NK cell-enriched splenocytes and activated Mϕ. CONCLUSION: Suppression of the growth of pulmonary tumour colonies by irradiations of mice with low-dose fractions of X-rays may result from stimulation of anti-tumour reactions mediated by NK cells and/or cytotoxic macrophages.
PURPOSE: The exploration of immune mechanisms of the tumour-inhibitory effect of exposures to low-level fractions of X-rays. MATERIALS AND METHODS: BALB/c mice were exposed to whole-body daily irradiations with 0.01, 0.02, or 0.1 Gy X-rays per day for 5 days/week for two weeks. Then, mice were intravenously injected with L1 tumour cells, killed 14 days later, and neoplastic colonies were counted in the lungs. Natural killer (NK) cell-enriched splenocytes and activated peritoneal macrophages (Mϕ) were collected and cytotoxic activities of these cells against susceptible tumour targets were assayed. Concanamycin A (CMA) and antibody against the ligand for the Fas receptor (FasL) were used to inhibit the NK cell-mediated cytotoxicity. Production of nitric oxide (NO) was quantified using the Griess reagent. Secretion of interferon-γ (IFN-γ), interleukin-1β (IL-1β), interleukin-12 (IL-12), and tumour necrosis factor-α (TNF-α) was measured using the enzyme-linked immunosorbent assays. RESULTS: All the exposures to X-rays significantly reduced the number of the induced tumour colonies and enhanced cytotoxic properties of the NK cell-enriched splenocytes and activated Mϕ. CONCLUSION: Suppression of the growth of pulmonary tumour colonies by irradiations of mice with low-dose fractions of X-rays may result from stimulation of anti-tumour reactions mediated by NK cells and/or cytotoxic macrophages.
Authors: Veronica R Bruce; Steven A Belinsky; Katherine Gott; Yushi Liu; Thomas March; Bobby Scott; Julie Wilder Journal: Dose Response Date: 2012-11-22 Impact factor: 2.658
Authors: Marek K Janiak; Marta Wincenciak; Aneta Cheda; Ewa M Nowosielska; Edward J Calabrese Journal: Cancer Immunol Immunother Date: 2017-03-30 Impact factor: 6.968
Authors: Ewa M Nowosielska; Aneta Cheda; Robert Zdanowski; Sławomir Lewicki; Bobby R Scott; Marek K Janiak Journal: Radiat Environ Biophys Date: 2018-04-06 Impact factor: 1.925