| Literature DB >> 21064230 |
Theresa J Ochoa1, Fulton P Rivera, Maria Bernal, Rina Meza, Lucie Ecker, Ana I Gil, David Cepeda, Susan Mosquito, Erik Mercado, Ryan C Maves, Eric R Hall, Ann-Mari Svennerholm, Annette McVeigh, Stephen Savarino, Claudio F Lanata.
Abstract
We analyzed a randomly selected group of 30 diffusely adherent (DAEC), 30 enteropathogenic, 30 enteroaggregative, and five Shiga toxin-producing Escherichia coli strains isolated from children with diarrhea. Enterotoxigenic E. coli (ETEC) colonization factors (CFs) were evaluated by a dot-blot assay using 21 CF-specific monoclonal antibodies. Out of 95 non-ETEC strains, three DAEC were found to express coli surface antigen 20 (CS20). No other E. coli expressed CFs. We confirmed the three CS20-positive strains as ETEC-negative by repeat PCR and as toxin-negative by ganglioside-GM1-enzyme-linked immunosorbent assay. To our knowledge, this is the first study that has identified currently recognized CFs in non-ETEC diarrheagenic E. coli strains identified using molecular methods. CFs may be an unrecognized relevant adherence factor in other E. coli, which may then play a role in pathogenesis and the immune response of the host.Entities:
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Year: 2010 PMID： 21064230 PMCID： PMC3170001 DOI： 10.1111/j.1574-695X.2010.00730.x
Source DB: PubMed Journal: FEMS Immunol Med Microbiol ISSN： 0928-8244