PURPOSE: To evaluate the sequence variations in the enhancer II (EnhII)/basal core promotor (BCP)/precore (PC) and X genes of hepatitis B virus (HBV) in Thai patients with hepatocellular carcinoma (HCC) by conducting a cross-sectional case-control study. METHODS: As much as 60 patients with HCC and 60 patients without HCC, who were matched for sex, age, hepatitis B e antigen (HBeAg) status, and HBV genotype, were included. Viral mutations in the EnhII/BCP/PC and X regions were characterized by direct sequencing in serum samples. RESULTS: The prevalence of T1753C/A, A1762T/G1764A and G1899A mutations were significantly higher in the HCC group compared to the non-HCC group (43.3 vs. 23.3%, P = 0.02; 88.3 vs. 53.0%, P < 0.001; and 35.0 vs. 8.3%, P = 0.001, respectively). No significant difference between groups was found with respect to G1613A, C1653T, C1766T/T1768A, A1846T/C, T1858C, and G1896A mutations. By multiple logistic regression analysis, the presence of cirrhosis, A1762T/G1764A and G1899A mutations were independently associated with the risk of HCC. CONCLUSION: These data suggested that A1762T/G1764A and G1899A mutations were associated with the development of HCC in Thai patients.
PURPOSE: To evaluate the sequence variations in the enhancer II (EnhII)/basal core promotor (BCP)/precore (PC) and X genes of hepatitis B virus (HBV) in Thai patients with hepatocellular carcinoma (HCC) by conducting a cross-sectional case-control study. METHODS: As much as 60 patients with HCC and 60 patients without HCC, who were matched for sex, age, hepatitis B e antigen (HBeAg) status, and HBV genotype, were included. Viral mutations in the EnhII/BCP/PC and X regions were characterized by direct sequencing in serum samples. RESULTS: The prevalence of T1753C/A, A1762T/G1764A and G1899A mutations were significantly higher in the HCC group compared to the non-HCC group (43.3 vs. 23.3%, P = 0.02; 88.3 vs. 53.0%, P < 0.001; and 35.0 vs. 8.3%, P = 0.001, respectively). No significant difference between groups was found with respect to G1613A, C1653T, C1766T/T1768A, A1846T/C, T1858C, and G1896A mutations. By multiple logistic regression analysis, the presence of cirrhosis, A1762T/G1764A and G1899A mutations were independently associated with the risk of HCC. CONCLUSION: These data suggested that A1762T/G1764A and G1899A mutations were associated with the development of HCC in Thai patients.
Entities:
Keywords:
Core promoter; Enhancer II; Hepatitis B virus; Hepatocellular carcinoma; Precore; X genes
Authors: Shuang-Yuan Kuang; Peta E Jackson; Jin-Bing Wang; Pei-Xing Lu; Alvaro Muñoz; Geng-Sun Qian; Thomas W Kensler; John D Groopman Journal: Proc Natl Acad Sci U S A Date: 2004-02-27 Impact factor: 11.205
Authors: Alexander Augusto Martinez; Yamitzel Zaldivar; Chen Ch Hong; Monica Viviana Alvarado-Mora; Rebecca Smith; Alma Y Ortiz; João Renato Rebello Pinho; Juan Cristina; Juan Miguel Pascale Journal: Mem Inst Oswaldo Cruz Date: 2013-08 Impact factor: 2.743