| Literature DB >> 21062640 |
Subrata Pandit1, Pulok K Mukherjee, Sivasankaran Ponnusankar, Murugan Venkatesh, N Srikanth.
Abstract
The present study was aimed to investigate the possible interaction of the standardized extract of Acorus calamus (AC) with Cytochrome P450 enzyme, quantitative determination of the α-asarone in the AC rhizome was performed by RP-HPLC method. In vitro interaction of the plant extract was evaluated by CYP450-carbon monoxide complex (CYP450-CO) assay. Effect on individual isoforms such as CYP3A4 and CYP2D6 isozymes were analyzed through fluorescence product formation and respective IC(50) values were determined. CYP450-CO assay showed moderate interaction potential. Extract showed higher IC(50) values (46.84±1.83-32.99±2.21 μg/ml) comparing to the standard inhibitors and lower IC(50) value than α-asarone (65.16±2.37-42.15±2.45 μg/ml).Entities:
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Year: 2010 PMID： 21062640 DOI： 10.1016/j.fitote.2010.11.009
Source DB: PubMed Journal: Fitoterapia ISSN： 0367-326X Impact factor: 2.882