| Literature DB >> 21059341 |
Nami Miyanishi1, Yukino Suzuki, Siro Simizu, Yoshiko Kuwabara, Kouji Banno, Kazuo Umezawa.
Abstract
Ovarian carcinomas are often highly invasive, especially in the peritoneal cavity; however, the mechanism involved in invasion is not yet fully understood. In the present research, we studied the role of NF-κB in the invasiveness of ovarian carcinoma cells by using (-)-DHMEQ, a specific inhibitor of NF-κB. (-)-DHMEQ inhibited invasion in vitro and the expression of CXCL12 and CXCR4. We found that neutralizing antibody against CXCR4 or knockdown of CXCR4 suppressed the invasion. Proteomic analysis revealed that CXCR4-siRNA treatment lowered the secretion of several invasion-related proteins, such as MMP-9 and uPA. These data imply that (-)-DHMEQ suppressed ovarian cell invasion via inhibition of the NF-κB-regulated autocrine system of CXCL12-CXCR4.Entities:
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Year: 2010 PMID： 21059341 DOI： 10.1016/j.bbrc.2010.11.007
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575