Popeye strikes again: The deep proteome of spinach leaves.

The cytoplasmic proteome of spinach leaves (Spinacia oleracea L) has been investigated with the help of commercially available (ProteoMiner) combinatorial peptide ligand libraries and with home-made ligand beads as prepared in our laboratory. The protein capture had been performed at three pH values (4.0, 7.0 and 9.3) and elution performed in 4% boiling SDS, 20mM DTT. The total number of unique gene products identified amounts to 322 proteins, of which 114 are in common with the control, untreated sample, 18 are present only in the control and 190 represent the new species detected with the help of all combined eluates and likely represent low-abundance species. This is the first in depth exploration of the spinach cytoplasmic proteome and might enable further studies on interaction, regulation and expression of proteins biological processes in combination or not with transcriptomics data.