Literature DB >> 21056038

Consequences of IkappaB alpha hydroxylation by the factor inhibiting HIF (FIH).

Ingrid L Devries1, Rachel J Hampton-Smith, Melinda M Mulvihill, Vera Alverdi, Daniel J Peet, Elizabeth A Komives.   

Abstract

The factor inhibiting HIF-1 (FIH-1) hydroxylates many ankyrin repeat-containing proteins including IκBα. It is widely speculated that hydroxylation of IκBα has functional consequences, but the effects of hydroxylation have not been demonstrated. We prepared hydroxylated IκBα and compared it to the unhydroxylated protein. Urea denaturation and amide H/D exchange experiments showed no change in the "foldedness" upon hydroxylation. Surface plasmon resonance measurements of binding to NFκB showed no difference in the NFκB binding kinetics or thermodynamics. Ubiquitin-independent proteasomal degradation experiments showed no difference in the half-life of the protein. Thus, it appears that hydroxylation of IκBα by FIH-1 is inconsequential, at least for the functions we could assay in vitro.
Copyright © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 21056038      PMCID： PMC3034650          DOI： 10.1016/j.febslet.2010.10.060

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  31 in total

1.  Protein folding and stability using denaturants.

Authors:  Timothy O Street; Naomi Courtemanche; Doug Barrick
Journal:  Methods Cell Biol       Date:  2008       Impact factor: 1.441

2.  Stabilizing IkappaBalpha by "consensus" design.

Authors:  Diego U Ferreiro; Carla F Cervantes; Stephanie M E Truhlar; Samuel S Cho; Peter G Wolynes; Elizabeth A Komives
Journal:  J Mol Biol       Date:  2006-11-15       Impact factor: 5.469

3.  Regions of IkappaBalpha that are critical for its inhibition of NF-kappaB.DNA interaction fold upon binding to NF-kappaB.

Authors:  Stephanie M E Truhlar; Justin W Torpey; Elizabeth A Komives
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-05       Impact factor: 11.205

4.  Hypoxia induces cyclooxygenase-2 via the NF-kappaB p65 transcription factor in human vascular endothelial cells.

Authors:  J F Schmedtje; Y S Ji; W L Liu; R N DuBois; M S Runge
Journal:  J Biol Chem       Date:  1997-01-03       Impact factor: 5.157

5.  Measurement of amide hydrogen exchange by MALDI-TOF mass spectrometry.

Authors:  J G Mandell; A M Falick; E A Komives
Journal:  Anal Chem       Date:  1998-10-01       Impact factor: 6.986

6.  ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism.

Authors:  James E Ferguson; Yaxu Wu; Kevin Smith; Peter Charles; Kyle Powers; Hong Wang; Cam Patterson
Journal:  Mol Cell Biol       Date:  2007-07-16       Impact factor: 4.272

7.  Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH).

Authors:  Matthew E Cockman; David E Lancaster; Ineke P Stolze; Kirsty S Hewitson; Michael A McDonough; Mathew L Coleman; Charlotte H Coles; Xiaohong Yu; Ronald T Hay; Steven C Ley; Christopher W Pugh; Neil J Oldham; Norma Masson; Christopher J Schofield; Peter J Ratcliffe
Journal:  Proc Natl Acad Sci U S A       Date:  2006-09-26       Impact factor: 11.205

8.  Expansion of protein domain repeats.

Authors:  Asa K Björklund; Diana Ekman; Arne Elofsson
Journal:  PLoS Comput Biol       Date:  2006-07-14       Impact factor: 4.475

9.  A homeostatic model of IkappaB metabolism to control constitutive NF-kappaB activity.

Authors:  Ellen L O'Dea; Derren Barken; Raechel Q Peralta; Kim T Tran; Shannon L Werner; Jeffrey D Kearns; Andre Levchenko; Alexander Hoffmann
Journal:  Mol Syst Biol       Date:  2007-05-08       Impact factor: 11.429

10.  Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor.

Authors:  Mathew L Coleman; Michael A McDonough; Kirsty S Hewitson; Charlotte Coles; Jasmin Mecinovic; Mariola Edelmann; Kristina M Cook; Matthew E Cockman; David E Lancaster; Benedikt M Kessler; Neil J Oldham; Peter J Ratcliffe; Christopher J Schofield
Journal:  J Biol Chem       Date:  2007-06-15       Impact factor: 5.157
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  11 in total

1.  Factor inhibiting HIF (FIH) recognizes distinct molecular features within hypoxia-inducible factor-α (HIF-α) versus ankyrin repeat substrates.

Authors:  Sarah E Wilkins; Sarah Karttunen; Rachel J Hampton-Smith; Iain Murchland; Anne Chapman-Smith; Daniel J Peet
Journal:  J Biol Chem       Date:  2012-01-23       Impact factor: 5.157

2.  MicroRNA-455 regulates brown adipogenesis via a novel HIF1an-AMPK-PGC1α signaling network.

Authors:  Hongbin Zhang; Meiping Guan; Kristy L Townsend; Tian Lian Huang; Ding An; Xu Yan; Ruidan Xue; Tim J Schulz; Jonathon Winnay; Marcelo Mori; Michael F Hirshman; Karsten Kristiansen; John S Tsang; Andrew P White; Aaron M Cypess; Laurie J Goodyear; Yu-Hua Tseng
Journal:  EMBO Rep       Date:  2015-08-24       Impact factor: 8.807

3.  Regulation of IL-1β-induced NF-κB by hydroxylases links key hypoxic and inflammatory signaling pathways.

Authors:  Carsten C Scholz; Miguel A S Cavadas; Murtaza M Tambuwala; Emily Hams; Javier Rodríguez; Alex von Kriegsheim; Philip Cotter; Ulrike Bruning; Padraic G Fallon; Alex Cheong; Eoin P Cummins; Cormac T Taylor
Journal:  Proc Natl Acad Sci U S A       Date:  2013-10-21       Impact factor: 11.205

Review 4.  The biological kinship of hypoxia with CSC and EMT and their relationship with deregulated expression of miRNAs and tumor aggressiveness.

Authors:  Bin Bao; Asfar S Azmi; Shadan Ali; Aamir Ahmad; Yiwei Li; Sanjeev Banerjee; Dejuan Kong; Fazlul H Sarkar
Journal:  Biochim Biophys Acta       Date:  2012-05-10

5.  Quantitative mass spectrometry reveals dynamics of factor-inhibiting hypoxia-inducible factor-catalyzed hydroxylation.

Authors:  Rachelle S Singleton; David C Trudgian; Roman Fischer; Benedikt M Kessler; Peter J Ratcliffe; Matthew E Cockman
Journal:  J Biol Chem       Date:  2011-07-30       Impact factor: 5.157

6.  FIH Regulates Cellular Metabolism through Hydroxylation of the Deubiquitinase OTUB1.

Authors:  Carsten C Scholz; Javier Rodriguez; Christina Pickel; Stephen Burr; Jacqueline-Alba Fabrizio; Karen A Nolan; Patrick Spielmann; Miguel A S Cavadas; Bianca Crifo; Doug N Halligan; James A Nathan; Daniel J Peet; Roland H Wenger; Alex Von Kriegsheim; Eoin P Cummins; Cormac T Taylor
Journal:  PLoS Biol       Date:  2016-01-11       Impact factor: 8.029

7.  Mint3/Apba3 depletion ameliorates severe murine influenza pneumonia and macrophage cytokine production in response to the influenza virus.

Authors:  Takayuki Uematsu; Tomoko Fujita; Hiroki J Nakaoka; Toshiro Hara; Noritada Kobayashi; Yoshinori Murakami; Motoharu Seiki; Takeharu Sakamoto
Journal:  Sci Rep       Date:  2016-11-24       Impact factor: 4.379

Review 8.  Hypoxia and Inflammation in Cancer, Focus on HIF and NF-κB.

Authors:  Laura D'Ignazio; Michael Batie; Sonia Rocha
Journal:  Biomedicines       Date:  2017-05-09

9.  Oxygen-dependent bond formation with FIH regulates the activity of the client protein OTUB1.

Authors:  Christina Pickel; Julia Günter; Amalia Ruiz-Serrano; Patrick Spielmann; Jacqueline-Alba Fabrizio; Witold Wolski; Daniel J Peet; Roland H Wenger; Carsten C Scholz
Journal:  Redox Biol       Date:  2019-07-02       Impact factor: 11.799

Review 10.  Hypoxia Induced NF-κB.

Authors:  Laura D'Ignazio; Sonia Rocha
Journal:  Cells       Date:  2016-03-08       Impact factor: 6.600
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