Literature DB >> 21053253

Genetic manipulation of Streptomyces species.

S Eric Nybo1, Micah D Shepherd, Mary A Bosserman, Jürgen Rohr.   

Abstract

This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR.

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Year:  2010        PMID： 21053253     DOI： 10.1002/9780471729259.mc10e03s19

Source DB:  PubMed          Journal:  Curr Protoc Microbiol


  6 in total

1.  Combination and improvement of conventional DNA extraction methods in Actinobacteria to obtain high-quantity and high-quality DNA.

Authors:  Zahra Khosravi Babadi; Abolfazl Narmani; Gholam Hossein Ebrahimipour; Joachim Wink
Journal:  Iran J Microbiol       Date:  2022-04

2.  An Efficient Method To Generate Gene Deletion Mutants of the Rapamycin-Producing Bacterium Streptomyces iranensis HM 35.

Authors:  Tina Netzker; Volker Schroeckh; Matthew A Gregory; Michal Flak; Mario K C Krespach; Peter F Leadlay; Axel A Brakhage
Journal:  Appl Environ Microbiol       Date:  2016-05-31       Impact factor: 4.792

3.  The Three Streptomyces lividans HtrA-Like Proteases Involved in the Secretion Stress Response Act in a Cooperative Manner.

Authors:  Rebeca L Vicente; Sonia Gullón; Silvia Marín; Rafael P Mellado
Journal:  PLoS One       Date:  2016-12-15       Impact factor: 3.240

4.  A novel two-component system involved in secretion stress response in Streptomyces lividans.

Authors:  Sonia Gullón; Rebeca L Vicente; Rafael P Mellado
Journal:  PLoS One       Date:  2012-11-14       Impact factor: 3.240

5.  Streptomyces sp. VN1, a producer of diverse metabolites including non-natural furan-type anticancer compound.

Authors:  Hue Thi Nguyen; Anaya Raj Pokhrel; Chung Thanh Nguyen; Van Thuy Thi Pham; Dipesh Dhakal; Haet Nim Lim; Hye Jin Jung; Tae-Su Kim; Tokutaro Yamaguchi; Jae Kyung Sohng
Journal:  Sci Rep       Date:  2020-02-04       Impact factor: 4.379

6.  Functional identification of a Streptomyces lividans FKBP-like protein involved in the folding of overproduced secreted proteins.

Authors:  R L Vicente; S Marín; J R Valverde; C Palomino; R P Mellado; S Gullón
Journal:  Open Biol       Date:  2019-10-30       Impact factor: 6.411
  6 in total

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