Literature DB >> 21051148

Multi-locus DNA sequencing of Toxoplasma gondii isolated from Brazilian pigs identifies genetically divergent strains.

E Frazão-Teixeira1, N Sundar, J P Dubey, M E Grigg, F C R de Oliveira.   

Abstract

Five Toxoplasma gondii isolates (TgPgBr1-5) were isolated from hearts and brains of pigs freshly purchased at the market of Campos dos Goytacazes, Northern Rio de Janeiro State, Brazil. Four of the five isolates were highly pathogenic in mice. Four genotypes were identified. Multi-locus PCR-DNA sequencing showed that each strain possessed a unique combination of archetypal and novel alleles not previously described in South America. The data suggest that different strains circulate in pigs destined for human consumption from those previously isolated from cats and chickens in Brazil. Further, multi-locus PCR-RFLP analyses failed to accurately genotype the Brazilian isolates due to the high presence of atypical alleles. This is the first report of multi-locus DNA sequencing of T. gondii isolates in pigs from Brazil.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID: 21051148      PMCID: PMC3141217          DOI: 10.1016/j.vetpar.2010.09.030

Source DB:  PubMed          Journal:  Vet Parasitol        ISSN: 0304-4017            Impact factor:   2.738


  32 in total

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4.  Isoenzyme analysis of 35 Toxoplasma gondii isolates and the biological and epidemiological implications.

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Journal:  J Parasitol       Date:  1992-10       Impact factor: 1.276

5.  Serological responses of equids fed Toxoplasma gondii oocysts.

Authors:  J P Dubey; G Desmonts
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6.  Biological and genetic characterisation of Toxoplasma gondii isolates from chickens (Gallus domesticus) from São Paulo, Brazil: unexpected findings.

Authors:  J P Dubey; D H Graham; C R Blackston; T Lehmann; S M Gennari; A M A Ragozo; S M Nishi; S K Shen; O C H Kwok; D E Hill; P Thulliez
Journal:  Int J Parasitol       Date:  2002-01       Impact factor: 3.981

7.  Toxoplasma gondii comprises three clonal lineages: correlation of parasite genotype with human disease.

Authors:  D K Howe; L D Sibley
Journal:  J Infect Dis       Date:  1995-12       Impact factor: 5.226

8.  Toxoplasma gondii isolates of free-ranging chickens from Rio de Janeiro, Brazil: mouse mortality, genotype, and oocyst shedding by cats.

Authors:  J P Dubey; D H Graham; Daniele Seipel da Silva; T Lehmann; Lilian M G Bahia-Oliveira
Journal:  J Parasitol       Date:  2003-08       Impact factor: 1.276

9.  Duration of immunity to shedding of Toxoplasma gondii oocysts by cats.

Authors:  J P Dubey
Journal:  J Parasitol       Date:  1995-06       Impact factor: 1.276

10.  Highly endemic, waterborne toxoplasmosis in north Rio de Janeiro state, Brazil.

Authors:  Lílian Maria Garcia Bahia-Oliveira; Jeffrey L Jones; Juliana Azevedo-Silva; Cristiane C F Alves; Fernando Oréfice; David G Addiss
Journal:  Emerg Infect Dis       Date:  2003-01       Impact factor: 6.883

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  8 in total

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2.  First report of genotype #65 of Toxoplasma gondii in pigs.

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Review 3.  Population genetics of Toxoplasma gondii: new perspectives from parasite genotypes in wildlife.

Authors:  Jered M Wendte; Amanda K Gibson; Michael E Grigg
Journal:  Vet Parasitol       Date:  2011-07-20       Impact factor: 2.738

4.  Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil.

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Journal:  Parasitol Res       Date:  2021-01-08       Impact factor: 2.289

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Journal:  J Clin Microbiol       Date:  2013-01-02       Impact factor: 5.948

6.  CD8 T Cells and Toxoplasma gondii: A New Paradigm.

Authors:  Jason P Gigley; Rajarshi Bhadra; Imtiaz A Khan
Journal:  J Parasitol Res       Date:  2011-05-18

Review 7.  Investigating the Determinants of Toxoplasma gondii Prevalence in Meat: A Systematic Review and Meta-Regression.

Authors:  Simone Belluco; Marzia Mancin; Daniele Conficoni; Giulia Simonato; Mario Pietrobelli; Antonia Ricci
Journal:  PLoS One       Date:  2016-04-15       Impact factor: 3.240

8.  Overlapping Toxoplasma gondii genotypes circulating in domestic animals and humans in Southeastern Brazil.

Authors:  Letícia A Silva; Renata O Andrade; Ana Carolina A V Carneiro; Ricardo W A Vitor
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  8 in total

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