Literature DB >> 21049123

Fluorogenic assay and live cell imaging of HIV-1 protease activity using acid-stable quantum dot-peptide complex.

Youngseon Choi1, Junghan Lee, Keumhyun Kim, Heeyeon Kim, Peter Sommer, Rita Song.   

Abstract

A novel QD-peptide complex for detecting HIV-1 protease activity was prepared from simple one step electrostatic interaction. Fluorescence recovery of the pre-quenched QD through fluorescence resonance energy transfer allowed for in vitro assay and live cell imaging of the protease activity in HIV-1 transfected cells, proving the potential for cell-based protease inhibitor screening.

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Year:  2010        PMID： 21049123     DOI： 10.1039/c0cc02702b

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  5 in total

1.  Designing Fluorescent Peptide Sensors with Dual Specificity for the Detection of HIV-1 Protease.

Authors:  Karla-Luise Herpoldt; Arbel Artzy-Schnirman; Andrew J Christofferson; Adam J Makarucha; Roberto de la Rica; Irene Yarovsky; Molly M Stevens
Journal:  Chem Mater       Date:  2015-10-06       Impact factor: 9.811

2.  Proteolytic activity at quantum dot-conjugates: kinetic analysis reveals enhanced enzyme activity and localized interfacial "hopping".

Authors:  W Russ Algar; Anthony Malonoski; Jeffrey R Deschamps; Juan B Blanco-Canosa; Kimihiro Susumu; Michael H Stewart; Brandy J Johnson; Philip E Dawson; Igor L Medintz
Journal:  Nano Lett       Date:  2012-06-25       Impact factor: 12.262

Review 3.  Different Approaches to Develop Nanosensors for Diagnosis of Diseases.

Authors:  Nina Arndt; Huong D N Tran; Run Zhang; Zhi Ping Xu; Hang T Ta
Journal:  Adv Sci (Weinh)       Date:  2020-10-28       Impact factor: 16.806

Review 4.  Recent advances in nanoparticle-based Förster resonance energy transfer for biosensing, molecular imaging and drug release profiling.

Authors:  Nai-Tzu Chen; Shih-Hsun Cheng; Ching-Ping Liu; Jeffrey S Souris; Chen-Tu Chen; Chung-Yuan Mou; Leu-Wei Lo
Journal:  Int J Mol Sci       Date:  2012-12-05       Impact factor: 5.923

Review 5.  QD-Based FRET Probes at a Glance.

Authors:  Armen Shamirian; Aashima Ghai; Preston T Snee
Journal:  Sensors (Basel)       Date:  2015-06-04       Impact factor: 3.576
  5 in total

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