Megan Mitchell1, Hassan W Bakos, Michelle Lane. 1. School of Paediatrics and Reproductive Health, The Robinson Institute, University of Adelaide, South Australia, Australia. megan.mitchell@adelaide.edu.au
Abstract
OBJECTIVE: To use a rodent model of male diet-induced obesity (DIO) to examine resultant preimplantation embryo development and implantation rate, as well as fetal and placental growth. DESIGN: Experimental animal study. SETTING: University research facilities. ANIMAL(S): C57BL/6 male and CBAxC57BL/6 female mice. INTERVENTION(S): Male mice were fed a standard rodent chow (lean) or a high-fat diet (obese) for up to 13 weeks. After mating, zygotes were collected and cultured to the blastocyst stage, then assessed or transferred into recipient females. MAIN OUTCOME MEASURE(S): Embryo morphology and cell number were assessed and pregnancy outcomes determined at postmortem day 18. RESULT(S): Embryos from obese males had reduced cleavage and decreased development to blastocyst stage during culture relative to control males. Blastocysts from obese males implanted at a reduced rate, and the proportion of fetuses that developed was significantly decreased, although fetal and placental weight did not differ between groups. CONCLUSION(S): This study demonstrates that paternal obesity impairs preimplantation embryo development and implantation but does not influence gross fetal or placental morphology. It highlights the important contribution that paternal health and lifestyle choices have for achieving a viable pregnancy.
OBJECTIVE: To use a rodent model of male diet-induced obesity (DIO) to examine resultant preimplantation embryo development and implantation rate, as well as fetal and placental growth. DESIGN: Experimental animal study. SETTING: University research facilities. ANIMAL(S): C57BL/6 male and CBAxC57BL/6 female mice. INTERVENTION(S): Male mice were fed a standard rodent chow (lean) or a high-fat diet (obese) for up to 13 weeks. After mating, zygotes were collected and cultured to the blastocyst stage, then assessed or transferred into recipient females. MAIN OUTCOME MEASURE(S): Embryo morphology and cell number were assessed and pregnancy outcomes determined at postmortem day 18. RESULT(S): Embryos from obese males had reduced cleavage and decreased development to blastocyst stage during culture relative to control males. Blastocysts from obese males implanted at a reduced rate, and the proportion of fetuses that developed was significantly decreased, although fetal and placental weight did not differ between groups. CONCLUSION(S): This study demonstrates that paternal obesity impairs preimplantation embryo development and implantation but does not influence gross fetal or placental morphology. It highlights the important contribution that paternal health and lifestyle choices have for achieving a viable pregnancy.
Authors: Tod Fullston; Helana Shehadeh; Lauren Y Sandeman; Wan Xian Kang; Linda L Wu; Rebecca L Robker; Nicole O McPherson; Michelle Lane Journal: J Assist Reprod Genet Date: 2015-04-09 Impact factor: 3.412
Authors: Upasna Sharma; Colin C Conine; Jeremy M Shea; Ana Boskovic; Alan G Derr; Xin Y Bing; Clemence Belleannee; Alper Kucukural; Ryan W Serra; Fengyun Sun; Lina Song; Benjamin R Carone; Emiliano P Ricci; Xin Z Li; Lucas Fauquier; Melissa J Moore; Robert Sullivan; Craig C Mello; Manuel Garber; Oliver J Rando Journal: Science Date: 2015-12-31 Impact factor: 47.728