Literature DB >> 21047535

A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing.

Iñaki Iturbe-Ormaetxe1, Megan Woolfit, Edwige Rancès, Anne Duplouy, Scott L O'Neill.   

Abstract

Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 21047535     DOI： 10.1016/j.mimet.2010.10.019

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  14 in total

1.  Wolbachia small noncoding RNAs and their role in cross-kingdom communications.

Authors:  Jaime G Mayoral; Mazhar Hussain; D Albert Joubert; Iñaki Iturbe-Ormaetxe; Scott L O'Neill; Sassan Asgari
Journal:  Proc Natl Acad Sci U S A       Date:  2014-12-15       Impact factor: 11.205

Review 2.  Wolbachia and the biological control of mosquito-borne disease.

Authors:  Iñaki Iturbe-Ormaetxe; Thomas Walker; Scott L O' Neill
Journal:  EMBO Rep       Date:  2011-05-06       Impact factor: 8.807

3.  Discovery of putative small non-coding RNAs from the obligate intracellular bacterium Wolbachia pipientis.

Authors:  Megan Woolfit; Manjula Algama; Jonathan M Keith; Elizabeth A McGraw; Jean Popovici
Journal:  PLoS One       Date:  2015-03-04       Impact factor: 3.240

4.  Reconstructing the functions of endosymbiotic Mollicutes in fungus-growing ants.

Authors:  Panagiotis Sapountzis; Mariya Zhukova; Jonathan Z Shik; Morten Schiott; Jacobus J Boomsma
Journal:  Elife       Date:  2018-11-20       Impact factor: 8.140

5.  Draft genome sequence of the male-killing Wolbachia strain wBol1 reveals recent horizontal gene transfers from diverse sources.

Authors:  Anne Duplouy; Iñaki Iturbe-Ormaetxe; Scott A Beatson; Jan M Szubert; Jeremy C Brownlie; Conor J McMeniman; Elizabeth A McGraw; Gregory D D Hurst; Sylvain Charlat; Scott L O'Neill; Megan Woolfit
Journal:  BMC Genomics       Date:  2013-01-16       Impact factor: 3.969

6.  Population genomics of the Wolbachia endosymbiont in Drosophila melanogaster.

Authors:  Mark F Richardson; Lucy A Weinert; John J Welch; Raquel S Linheiro; Michael M Magwire; Francis M Jiggins; Casey M Bergman
Journal:  PLoS Genet       Date:  2012-12-20       Impact factor: 5.917

7.  Targeted genome enrichment for efficient purification of endosymbiont DNA from host DNA.

Authors:  Sandrine Geniez; Jeremy M Foster; Sanjay Kumar; Bouziane Moumen; Emily Leproust; Owen Hardy; Moraima Guadalupe; Stephen J Thomas; Braden Boone; Cynthia Hendrickson; Didier Bouchon; Pierre Grève; Barton E Slatko
Journal:  Symbiosis       Date:  2013-01-06       Impact factor: 2.268

8.  Comparative genomics of Wolbachia and the bacterial species concept.

Authors:  Kirsten Maren Ellegaard; Lisa Klasson; Kristina Näslund; Kostas Bourtzis; Siv G E Andersson
Journal:  PLoS Genet       Date:  2013-04-04       Impact factor: 5.917

9.  Genomic evolution of the pathogenic Wolbachia strain, wMelPop.

Authors:  Megan Woolfit; Iñaki Iturbe-Ormaetxe; Jeremy C Brownlie; Thomas Walker; Markus Riegler; Andrei Seleznev; Jean Popovici; Edwige Rancès; Bryan A Wee; Jennifer Pavlides; Mitchell J Sullivan; Scott A Beatson; Amanda Lane; Manpreet Sidhu; Conor J McMeniman; Elizabeth A McGraw; Scott L O'Neill
Journal:  Genome Biol Evol       Date:  2013       Impact factor: 3.416

10.  Wolbachia variants induce differential protection to viruses in Drosophila melanogaster: a phenotypic and phylogenomic analysis.

Authors:  Ewa Chrostek; Marta S P Marialva; Sara S Esteves; Lucy A Weinert; Julien Martinez; Francis M Jiggins; Luis Teixeira
Journal:  PLoS Genet       Date:  2013-12-12       Impact factor: 5.917
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