BACKGROUND: While some recently transmitted HIV clade C (HIV-C) strains exhibited tier 1 neutralization phenotypes, most were tier 2 strains (J Virol 2010; 84:1439). Because induction of neutralizing antibodies (nAbs) through vaccination against tier 2 viruses has proven difficult, we have generated a tier 1, clade C simian-human immunodeficiency virus (SHIV-C) to permit efficacy testing of candidate AIDS vaccines against tier 1 viruses. METHODS: SHIV-1157ipEL was created by swapping env of a late-stage virus with that of a tier 1, early form. RESULTS: After adaptation to rhesus macaques (RM), passaged SHIV-1157ipEL-p replicated vigorously in vitro and in vivo while maintaining R5 tropism. The virus was reproducibly transmissible intrarectally. Phylogenetically, SHIV-1157ipEL-p Env clustered with HIV-C sequences. All RM chronically infected with SHIV-1157ipEL-p developed high nAb titers against autologous as well as heterologous tier 1 strains. CONCLUSIONS: SHIV-1157ipEL-p was reproducibly transmitted in RM, induced cross-clade nAbs, and represents a tool to evaluate anti-HIV-C nAb responses in primates.
BACKGROUND: While some recently transmitted HIV clade C (HIV-C) strains exhibited tier 1 neutralization phenotypes, most were tier 2 strains (J Virol 2010; 84:1439). Because induction of neutralizing antibodies (nAbs) through vaccination against tier 2 viruses has proven difficult, we have generated a tier 1, clade C simian-human immunodeficiency virus (SHIV-C) to permit efficacy testing of candidate AIDS vaccines against tier 1 viruses. METHODS: SHIV-1157ipEL was created by swapping env of a late-stage virus with that of a tier 1, early form. RESULTS: After adaptation to rhesus macaques (RM), passaged SHIV-1157ipEL-p replicated vigorously in vitro and in vivo while maintaining R5 tropism. The virus was reproducibly transmissible intrarectally. Phylogenetically, SHIV-1157ipEL-p Env clustered with HIV-C sequences. All RM chronically infected with SHIV-1157ipEL-p developed high nAb titers against autologous as well as heterologous tier 1 strains. CONCLUSIONS: SHIV-1157ipEL-p was reproducibly transmitted in RM, induced cross-clade nAbs, and represents a tool to evaluate anti-HIV-C nAb responses in primates.
Authors: C A Derdeyn; J M Decker; J N Sfakianos; X Wu; W A O'Brien; L Ratner; J C Kappes; G M Shaw; E Hunter Journal: J Virol Date: 2000-09 Impact factor: 5.103
Authors: T Ndung'u; Y Lu; B Renjifo; N Touzjian; N Kushner; V Pena-Cruz; V A Novitsky; T H Lee; M Essex Journal: J Virol Date: 2001-12 Impact factor: 5.103
Authors: R Hofmann-Lehmann; R K Swenerton; V Liska; C M Leutenegger; H Lutz; H M McClure; R M Ruprecht Journal: AIDS Res Hum Retroviruses Date: 2000-09-01 Impact factor: 2.205
Authors: Mark Cayabyab; Daniela Rohne; Georgios Pollakis; Claudia Mische; Tseyanish Messele; Almaz Abebe; Bijan Etemad-Moghadam; Peter Yang; Scott Henson; Michael Axthelm; Jaap Goudsmit; Norman L Letvin; Joseph Sodroski Journal: AIDS Res Hum Retroviruses Date: 2004-01 Impact factor: 2.205
Authors: Delphine C Malherbe; Jason Mendy; Lo Vang; Philip T Barnette; Jason Reed; Samir K Lakhashe; Joshua Owuor; Johannes S Gach; Alfred W Legasse; Michael K Axthelm; Celia C LaBranche; David Montefiori; Donald N Forthal; Byung Park; James M Wilson; James H McLinden; Jinhua Xiang; Jack T Stapleton; Jonah B Sacha; Barton F Haynes; Hua-Xin Liao; Ruth M Ruprecht; Jonathan Smith; Marc Gurwith; Nancy L Haigwood; Jeff Alexander Journal: J Virol Date: 2018-01-02 Impact factor: 5.103
Authors: Josephine H Cox; Maria G Ferrari; Patricia Earl; James R Lane; Linda L Jagodzinski; Victoria R Polonis; Ellen G Kuta; Jean D Boyer; Silvia Ratto-Kim; Leigh-Anne Eller; Doan-Trang Pham; Lydia Hart; David Montefiori; Guido Ferrari; Stephanie Parrish; David B Weiner; Bernard Moss; Jerome H Kim; Deborah Birx; Thomas C VanCott Journal: Vaccine Date: 2012-01-09 Impact factor: 3.641
Authors: Barbara C Bachler; Michael Humbert; Brisa Palikuqi; Nagadenahalli B Siddappa; Samir K Lakhashe; Robert A Rasmussen; Ruth M Ruprecht Journal: J Virol Date: 2013-02-06 Impact factor: 5.103
Authors: Dan H Barouch; Kathryn E Stephenson; Erica N Borducchi; Kaitlin Smith; Kelly Stanley; Anna G McNally; Jinyan Liu; Peter Abbink; Lori F Maxfield; Michael S Seaman; Anne-Sophie Dugast; Galit Alter; Melissa Ferguson; Wenjun Li; Patricia L Earl; Bernard Moss; Elena E Giorgi; James J Szinger; Leigh Anne Eller; Erik A Billings; Mangala Rao; Sodsai Tovanabutra; Eric Sanders-Buell; Mo Weijtens; Maria G Pau; Hanneke Schuitemaker; Merlin L Robb; Jerome H Kim; Bette T Korber; Nelson L Michael Journal: Cell Date: 2013-10-24 Impact factor: 41.582
Authors: Barbara C Bachler; Michael Humbert; Samir K Lakhashe; Robert A Rasmussen; Ruth M Ruprecht Journal: Retrovirology Date: 2013-06-21 Impact factor: 4.602
Authors: Debashis Dutta; Samuel Johnson; Alisha Dalal; Martin J Deymier; Eric Hunter; Siddappa N Byrareddy Journal: PLoS One Date: 2018-05-14 Impact factor: 3.240