Literature DB >> 21043058

Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells.

Shao-zhi Zhang1, Huan Qian, Zhen Wang, Ju-li Fan, Qian Zhou, Guang-ming Chen, Rui Li, Shan Fu, Jie Sun.   

Abstract

Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells (hBM-MSCs) will facilitate their application in medical treatment and bioengineering. A pilot study on the freeze-drying of hBM-MSCs was carried out. hBM-MSCs were loaded with trehalose. The glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying experiment. After freeze-drying, various rehydration processes were tested. The highest recovery rate of hBM-MSCs was (69.33±13.08)%. Possible methods to improve freeze-drying outcomes are discussed. In conclusion, the present study has laid a foundation for the freeze-drying hBM-MSCs.

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Year:  2010        PMID： 21043058      PMCID： PMC2970899          DOI： 10.1631/jzus.B1000184

Source DB:  PubMed          Journal:  J Zhejiang Univ Sci B        ISSN： 1673-1581            Impact factor:   3.066


  19 in total

Review 1.  The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state.

Authors:  J H Crowe; L M Crowe; A E Oliver; N Tsvetkova; W Wolkers; F Tablin
Journal:  Cryobiology       Date:  2001-09       Impact factor: 2.487

2.  Intracellular delivery of trehalose into mammalian cells by electropermeabilization.

Authors:  R Shirakashi; C M Köstner; K J Müller; M Kürschner; U Zimmermann; V L Sukhorukov
Journal:  J Membr Biol       Date:  2002-09-01       Impact factor: 1.843

Review 3.  Constraints of tolerance: why are desiccation-tolerant organisms so small or rare?

Authors:  Peter Alpert
Journal:  J Exp Biol       Date:  2006-05       Impact factor: 3.312

4.  Bone marrow-derived human mesenchymal stem cells become quiescent on soft substrates but remain responsive to chemical or mechanical stimuli.

Authors:  Jessamine P Winer; Paul A Janmey; Margaret E McCormick; Makoto Funaki
Journal:  Tissue Eng Part A       Date:  2009-01       Impact factor: 3.845

5.  Evaluation of bone marrow mesenchymal stem cell standard cryopreservation procedure efficiency.

Authors:  K A T Carvalho; C C Cury; L Oliveira; R I I Cattaned; M Malvezzi; J C Francisco; A Pachalok; M Olandoski; J R Faria-Neto; L C Guarita-Souza
Journal:  Transplant Proc       Date:  2008-04       Impact factor: 1.066

6.  Beneficial effect of intracellular trehalose on the membrane integrity of dried mammalian cells.

Authors:  T Chen; J P Acker; A Eroglu; S Cheley; H Bayley; A Fowler; M Toner
Journal:  Cryobiology       Date:  2001-09       Impact factor: 2.487

7.  Morphology study of freeze-drying mononuclear cells of human cord blood.

Authors:  Jun Li; Tse-Chao Hua; Xue-Lian Gu; Yu Ding; Ming Luo; Hong-Hai Xiao; Zhi-Jiang Wu; Lv-Rong Meng; Qi-Rong Gao; Jian Chen
Journal:  Cryo Letters       Date:  2005 May-Jun       Impact factor: 1.066

8.  Improved preservation of human red blood cells by lyophilization.

Authors:  Ying Han; Guo Bo Quan; Xiu Zhen Liu; En Pu Ma; An Liu; Peng Jin; Wei Cao
Journal:  Cryobiology       Date:  2005-10       Impact factor: 2.487

9.  Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells.

Authors:  Ying Xiang; Qiang Zheng; Bing-bing Jia; Guo-ping Huang; Yu-lin Xu; Jin-fu Wang; Zhi-jun Pan
Journal:  J Zhejiang Univ Sci B       Date:  2007-02       Impact factor: 3.066

10.  Beneficial effect of microinjected trehalose on the cryosurvival of human oocytes.

Authors:  Ali Eroglu; Mehmet Toner; Thomas L Toth
Journal:  Fertil Steril       Date:  2002-01       Impact factor: 7.329
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  7 in total

Review 1.  Cryopreservation of NK and T Cells Without DMSO for Adoptive Cell-Based Immunotherapy.

Authors:  Xue Yao; Sandro Matosevic
Journal:  BioDrugs       Date:  2021-08-24       Impact factor: 5.807

2.  Human Placental Mesenchymal Stem Cells for the Treatment of ARDS in Rat.

Authors:  Zurab Kakabadze; Nicholas Kipshidze; Teona Paresishvili; Nodar Kipshidze; Zurab Vadachkoria; David Chakhunashvili
Journal:  Stem Cells Int       Date:  2022-06-22       Impact factor: 5.131

Review 3.  Intracellular Delivery of Trehalose for Cell Banking.

Authors:  Samantha Stewart; Xiaoming He
Journal:  Langmuir       Date:  2018-08-20       Impact factor: 3.882

4.  Stabilization of Transfected Cells Expressing Low-Incidence Blood Group Antigens: Novel Methods Facilitating Their Use as Reagent-Cells.

Authors:  Cecilia González; Rosa Esteban; Carme Canals; Eduardo Muñiz-Díaz; Núria Nogués
Journal:  PLoS One       Date:  2016-09-07       Impact factor: 3.240

Review 5.  Recent Advances and Future Direction in Lyophilisation and Desiccation of Mesenchymal Stem Cells.

Authors:  Akalabya Bissoyi; Awanish Kumar; Albert A Rizvanov; Alexander Nesmelov; Oleg Gusev; Pradeep Kumar Patra; Arindam Bit
Journal:  Stem Cells Int       Date:  2016-08-14       Impact factor: 5.443

6.  Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors.

Authors:  Ann Kakabadze; Konstantine Mardaleishvili; George Loladze; Lia Karalashvili; Gocha Chutkerashvili; David Chakhunashvili; Zurab Kakabadze
Journal:  Oncol Lett       Date:  2017-01-25       Impact factor: 2.967

7.  Properties of viable lyopreserved amnion are equivalent to viable cryopreserved amnion with the convenience of ambient storage.

Authors:  Sandeep Dhall; Malathi Sathyamoorthy; Jin-Qiang Kuang; Tyler Hoffman; Matthew Moorman; Anne Lerch; Vimal Jacob; Steven Michael Sinclair; Alla Danilkovitch
Journal:  PLoS One       Date:  2018-10-02       Impact factor: 3.240
  7 in total

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