Literature DB >> 21041947

Using neutron protein crystallography to understand enzyme mechanisms.

Jenny P Glusker1, H L Carrell, Andrey Y Kovalevsky, Leif Hanson, S Zoe Fisher, Marat Mustyakimov, Sax Mason, Trevor Forsyth, Paul Langan.   

Abstract

A description is given of the results of neutron diffraction studies of the structures of four different metal-ion complexes of deuterated D-xylose isomerase. These represent four stages in the progression of the biochemical catalytic action of this enzyme. Analyses of the structural changes observed between the various three-dimensional structures lead to some insight into the mechanism of action of this enzyme.

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Year:  2010        PMID： 21041947      PMCID： PMC2967424          DOI： 10.1107/S0907444910027915

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  6 in total

1.  Locating active-site hydrogen atoms in D-xylose isomerase: time-of-flight neutron diffraction.

Authors:  Amy K Katz; Xinmin Li; H L Carrell; B Leif Hanson; Paul Langan; Leighton Coates; Benno P Schoenborn; Jenny P Glusker; Gerard J Bunick
Journal:  Proc Natl Acad Sci U S A       Date:  2006-05-17       Impact factor: 11.205

2.  X-ray analysis of D-xylose isomerase at 1.9 A: native enzyme in complex with substrate and with a mechanism-designed inactivator.

Authors:  H L Carrell; J P Glusker; V Burger; F Manfre; D Tritsch; J F Biellmann
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 11.205

3.  Metal ion roles and the movement of hydrogen during reaction catalyzed by D-xylose isomerase: a joint x-ray and neutron diffraction study.

Authors:  Andrey Y Kovalevsky; Leif Hanson; S Zoe Fisher; Marat Mustyakimov; Sax A Mason; V Trevor Forsyth; Matthew P Blakeley; David A Keen; Trixie Wagner; H L Carrell; Amy K Katz; Jenny P Glusker; Paul Langan
Journal:  Structure       Date:  2010-06-09       Impact factor: 5.006

4.  X-ray crystal structure of D-xylose isomerase at 4-A resolution.

Authors:  H L Carrell; B H Rubin; T J Hurley; J P Glusker
Journal:  J Biol Chem       Date:  1984-03-10       Impact factor: 5.157

5.  Hydrogen location in stages of an enzyme-catalyzed reaction: time-of-flight neutron structure of D-xylose isomerase with bound D-xylulose.

Authors:  Andrey Y Kovalevsky; Amy K Katz; H L Carrell; Leif Hanson; Marat Mustyakimov; S Zoe Fisher; Leighton Coates; Benno P Schoenborn; Gerard J Bunick; Jenny P Glusker; Paul Langan
Journal:  Biochemistry       Date:  2008-06-26       Impact factor: 3.162

6.  Direct determination of the protonation states of aspartic acid-102 and histidine-57 in the tetrahedral intermediate of the serine proteases: neutron structure of trypsin.

Authors:  A A Kossiakoff; S A Spencer
Journal:  Biochemistry       Date:  1981-10-27       Impact factor: 3.162
  6 in total
  1 in total

Review 1.  From Molecular Phylogenetics to Quantum Chemistry: Discovering Enzyme Design Principles through Computation.

Authors:  Troy Wymore; Charles L Brooks
Journal:  Comput Struct Biotechnol J       Date:  2012-11-30       Impact factor: 7.271
  1 in total

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