Salinicoccus sesuvii sp. nov., isolated from the rhizosphere of Sesuvium portulacastrum.

A Gram-staining-positive coccus, designated CC-SPL15-2(T), was isolated from the rhizosphere of Sesuvium portulacastrum. By 16S rRNA gene sequence analysis, it was shown that strain CC-SPL15-2(T) belonged to the genus Salinicoccus. The isolate was most closely related to Salinicoccus hispanicus DSM 5352(T) (98.3 % 16S rRNA gene sequence similarity) and Salinicoccus roseus DSM 5351(T) (96.7 %); similarities to all other members of the genus Salinicoccus were <96.5 %. In accordance with characteristics of the genus Salinicoccus, the quinone system was mainly composed of menaquinone MK-6. The polar lipid profile exhibited the major components diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. In the polyamine pattern, spermidine was the predominant compound. The fatty acids were anteiso-C(15 : 0), iso-C(15 : 0), iso-C(16 : 0) and anteiso-C(17 : 0), which supported the affiliation of strain CC-SPL15-2(T) to the genus Salinicoccus. DNA-DNA relatedness between strain CC-SPL15-2(T) and S. hispanicus CCUG 43288(T) was 42 and 32 % (reciprocal analysis). From these data as well as from physiological and biochemical tests, a clear differentiation of strain CC-SPL15-2(T) from S. hispanicus and other members of the genus Salinicoccus was possible. We propose that strain CC-SPL15-2(T) be assigned to a novel species, with the name Salinicoccus sesuvii sp. nov. The type strain is CC-SPL15-2(T) ( = DSM 23267(T)  = CCM 7756(T)).