| Literature DB >> 21036337 |
Elena Barbieri1, Paola Ceccaroli, Roberta Saltarelli, Chiara Guidi, Lucia Potenza, Marina Basaglia, Federico Fontana, Enrico Baldan, Sergio Casella, Ouafae Ryahi, Alessandra Zambonelli, Vilberto Stocchi.
Abstract
Diversity of nitrogen-fixing bacteria and the nitrogen-fixation activity was investigated in Tuber magnatum, the most well-known prized species of Italian white truffle. Degenerate PCR primers were applied to amplify the nitrogenase gene nifH from T. magnatum ascomata at different stages of maturation. Putative amino acid sequences revealed mainly the presence of Alphaproteobacteria belonging to Bradyrhizobium spp. and expression of nifH genes from Bradyrhizobia was detected. The nitrogenase activity evaluated by acetylene reduction assay was 0.5-7.5μmolC(2)H(4)h(-1)g(-1), comparable with early nodules of legumes associated with specific nitrogen-fixing bacteria. This is the first demonstration of nitrogenase expression gene and activity within truffle.Entities:
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Year: 2010 PMID： 21036337 DOI： 10.1016/j.funbio.2010.09.001
Source DB: PubMed Journal: Fungal Biol