Literature DB >> 21036133

PCPP (poly[di(carboxylatophenoxy)-phosphazene]) microparticles co-encapsulating ovalbumin and CpG oligo-deoxynucleotides are potent enhancers of antigen specific Th1 immune responses in mice.

Srinivas Garlapati1, Nelson F Eng, Heather L Wilson, Rachelle Buchanan, George K Mutwiri, Lorne A Babiuk, Volker Gerdts.   

Abstract

We generated poly[di(carboxylatophenoxy)-phosphazene] (PCPP) microparticles encapsulating ovalbumin (OVA) and CpG of 0.5-2.5 μm in diameter with an encapsulation efficiency of approximately 63% and 95% respectively. In mice the microparticles generated high antigen-specific IgG, IgG1 and IgG2a titers with higher IgG2a/IgG1 ratios. Whole body in vivo imaging of mice subcutaneously injected with MPs showed several fold increase of OVA and CpG in draining inguinal lymph nodes compared to soluble formulations. We conclude that PCPP MPs are more effective in enhancing immune responses compared to soluble formulations, due to co-delivery of OVA and CpG resulting in a Th1 type of immune response.
Copyright © 2010 Elsevier Ltd. All rights reserved.

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Year:  2010        PMID： 21036133     DOI： 10.1016/j.vaccine.2010.09.080

Source DB:  PubMed          Journal:  Vaccine        ISSN： 0264-410X            Impact factor:   3.641


  7 in total

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Authors:  Rachelle M Buchanan; Sherry Tetland; Heather L Wilson
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Review 6.  Evidence for a common mucosal immune system in the pig.

Authors:  Heather L Wilson; Milan R Obradovic
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Journal:  Front Immunol       Date:  2020-10-23       Impact factor: 7.561
  7 in total

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