T Lou1, X Xiang, D Wu. 1. Institute of Radiation Medicine, Academy of Military Medicine Science , Beijing 100850, P . R. China.
Abstract
BACKGROUND: To establish a model for the study of transformation of human cell by radon and its progeny. METHODS: BEP2D, a HPV18 immortalized human bronchial epithelial cell line, was irradiated with high-energy α-particles emitted by ²³⁸Pu. RESULTS: A single 1. 5 Gy dose of α-particles induced transformation of the cells. A series of sequential steps arose among transformed cells, including altered growth kinetics, resistance to serum-induced terminal differentiation, and anchorage-independence growth. CONCLUSIONS: This cell line may provide a model to study the cellular and molecular changes at various stages in radiation carcinogenesis.
BACKGROUND: To establish a model for the study of transformation of human cell by radon and its progeny. METHODS: BEP2D, a HPV18 immortalized human bronchial epithelial cell line, was irradiated with high-energy α-particles emitted by ²³⁸Pu. RESULTS: A single 1. 5 Gy dose of α-particles induced transformation of the cells. A series of sequential steps arose among transformed cells, including altered growth kinetics, resistance to serum-induced terminal differentiation, and anchorage-independence growth. CONCLUSIONS: This cell line may provide a model to study the cellular and molecular changes at various stages in radiation carcinogenesis.