| Literature DB >> 20981893 |
Rune Thomsen1, Christina Ane Elisabeth Sølvsten, Toke Elbek Linnet, Jenny Blechingberg, Anders Lade Nielsen.
Abstract
A common method for calculating results from qPCR experiments is the comparative Ct method, also called the 2(-ΔΔCt) method. However, several assumptions are included in the 2(-ΔΔCt) method and standard statistical analyses are not directly applicable. Here, we describe a different method, the X(0) method, for result calculations and statistical analysis from qPCR experiments. The X(0) method differs from the 2(-ΔΔCt) method by introducing a conversion of the exponentially related Ct values into linearly related X(0) values, which represent the amount of starting material in a qPCR experiment. Results calculated by the X(0) method are illustrated for qPCR experiments with technical and biological replicates, including procedures to calculate standard deviations. Incorporation of primer efficiencies in calculations by the X(0) method is also described. Altogether, the X(0) method constitutes a very simple and accurate alternative to the 2(-ΔΔCt) method for result calculations from qPCR data.Mesh：
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Year: 2010 PMID： 20981893 DOI： 10.1142/s0219720010004963
Source DB: PubMed Journal: J Bioinform Comput Biol ISSN： 0219-7200 Impact factor: 1.122