Yan-bin Wang1, Zhao-xu Yang. 1. Department of Gastroenterology, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing 100050, China. maogou0806@sina.com
Abstract
OBJECTIVE: To investigate the effect of oxygen free radicals on intestinal mucosal barrier in traumatic brain injury rats. METHODS: A total of 64 health male Wistar rats were divided randomly into two groups: traumatic brain injury (TBI) (n=32); Control group with sham operation (n=32). Each group was divided into four subgroups at 6, 12, 24 and 48 h post-operation (n=8). The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were measured in intestinal mucosa, mesenteric lymph nodes, liver, spleen, pancreas, lung and kidney tissue. The marked bacilli were counted under a fluorescent microscope. RESULTS: The positive rates of bacterial translocation in TBI group were obviously higher than those of control group (6 h: 8.3% vs. 2.1%, 12 h: 25.0% vs. 2.1%, 24 h: 27.1% vs. 2.1%, 48 h: 12.5% vs. 0.0% , P < 0.05). The levels of MDA in TBI group were obviously higher than those of control group (6 h: 4.9 +/- 0.7 vs. 2.6 +/- 0.3, 12 h: 6.1 +/- 0.8 vs. 2.8 +/- 0.5, 24 h: 5.9 +/- 0.4 vs. 2.6 +/- 0.3, 48 h: 5.3 +/- 0.5 vs. 2.7 +/- 0.4 (nmol/mg pro), P < 0.05), and GSH (6 h: 287 +/- 36 vs. 408 +/- 253; 12 h: 192 +/- 25 vs. 421 +/- 46; 24 h: 160 +/- 48 vs. 432 +/- 35; 48 h: 241 +/- 31 vs. 394 +/- 51 (mg/g pro) P < 0.05). SOD (6 h: 19.5 +/- 3.3 vs. 21.1 +/- 1.9; 12 h: 11.8 +/- 2.6 vs. 20.7 +/- 5.3; 24 h: 13.3 +/- 3.3 vs. 20 +/- 3.3; 48 h: 15.1 +/- 1.5 vs. 21.2 +/- 3.2 (U/mg pro). P < 0.05) were lower than the control group at the same time point. CONCLUSIONS: Bacterial translocation occurs obviously in TBI rats. It indicates that oxygen free radicals play important roles in stress-related changes of intestinal mucosal barrier in early stage of traumatic brain injury.
OBJECTIVE: To investigate the effect of oxygen free radicals on intestinal mucosal barrier in traumatic brain injuryrats. METHODS: A total of 64 health male Wistar rats were divided randomly into two groups: traumatic brain injury (TBI) (n=32); Control group with sham operation (n=32). Each group was divided into four subgroups at 6, 12, 24 and 48 h post-operation (n=8). The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH) were measured in intestinal mucosa, mesenteric lymph nodes, liver, spleen, pancreas, lung and kidney tissue. The marked bacilli were counted under a fluorescent microscope. RESULTS: The positive rates of bacterial translocation in TBI group were obviously higher than those of control group (6 h: 8.3% vs. 2.1%, 12 h: 25.0% vs. 2.1%, 24 h: 27.1% vs. 2.1%, 48 h: 12.5% vs. 0.0% , P < 0.05). The levels of MDA in TBI group were obviously higher than those of control group (6 h: 4.9 +/- 0.7 vs. 2.6 +/- 0.3, 12 h: 6.1 +/- 0.8 vs. 2.8 +/- 0.5, 24 h: 5.9 +/- 0.4 vs. 2.6 +/- 0.3, 48 h: 5.3 +/- 0.5 vs. 2.7 +/- 0.4 (nmol/mg pro), P < 0.05), and GSH (6 h: 287 +/- 36 vs. 408 +/- 253; 12 h: 192 +/- 25 vs. 421 +/- 46; 24 h: 160 +/- 48 vs. 432 +/- 35; 48 h: 241 +/- 31 vs. 394 +/- 51 (mg/g pro) P < 0.05). SOD (6 h: 19.5 +/- 3.3 vs. 21.1 +/- 1.9; 12 h: 11.8 +/- 2.6 vs. 20.7 +/- 5.3; 24 h: 13.3 +/- 3.3 vs. 20 +/- 3.3; 48 h: 15.1 +/- 1.5 vs. 21.2 +/- 3.2 (U/mg pro). P < 0.05) were lower than the control group at the same time point. CONCLUSIONS: Bacterial translocation occurs obviously in TBI rats. It indicates that oxygen free radicals play important roles in stress-related changes of intestinal mucosal barrier in early stage of traumatic brain injury.
Authors: Mauro Robson Torres de Castro; Ana Paula de Oliveira Ferreira; Guilherme Lago Busanello; Luís Roberto Hart da Silva; Mauro Eduardo Porto da Silveira Junior; Fernando da Silva Fiorin; Gabriela Arrifano; Maria Elena Crespo-López; Rômulo Pillon Barcelos; María J Cuevas; Guilherme Bresciani; Javier González-Gallego; Michele Rechia Fighera; Luiz Fernando Freire Royes Journal: J Physiol Date: 2017-07-30 Impact factor: 5.182