| Literature DB >> 20978975 |
Adam Charlton1, Michael Zachariou.
Abstract
Where an affinity tag has served its purpose it may become desirable to remove it from the protein of interest. This chapter describes the removal of such fusion partners from the intended protein product by cleavage with site-specific endoproteases. Methods to achieve proteolytic cleavage of the fusion proteins are provided, along with techniques for optimising the yield of authentic product.Mesh：
Substances：
Year: 2011 PMID： 20978975 DOI： 10.1007/978-1-60761-913-0_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745