Literature DB >> 20976525

Temporal trends of circulating nitric oxide and pro-inflammatory cytokine responses ex vivo in intra-abdominal sepsis: results from a cohort study.

Miriam Ojeda Ojeda1, Hilev Larrondo Muguercia, Abel Magdariaga Figuerola, Alfredo Sánchez Valdivia, Ingrid Rodríguez Alonso, Carmen Valenzuela Silva, Elizeth García Iglesias, Emma Domínguez Alonso, Wim A Buurman, Manuel de Jesús Araña Rosaínz.   

Abstract

OBJECTIVE AND
DESIGN: To evaluate the association of pro-inflammatory mediators with organ dysfunction and adverse outcome in intra-abdominal sepsis patients.
SUBJECTS: Twenty-one patients admitted to the Intensive Care Unit (ICU) were prospectively included in the study. Only patients with surgical diagnosis of intra-abdominal sepsis were enrolled.
RESULTS: Tumor necrosis factor-α (TNFα) and interleukin (IL)-6 produced ex vivo were significantly lower in non-survivors on admission (p = 0.021) and day 2 (p = 0.013), respectively. Nitric oxide (NO(x)) levels were significantly higher in non-survivors from the onset of sepsis and until day 4 after diagnosis (p < 0.05). Circulating lymphocyte counts were lower in non-survivors after admission over time, but there was no association with impaired cytokine production in this group of patients during the entire follow-up. All non-survivors developed nosocomial pneumonia concomitantly with multiple organ dysfunction and septic shock. There was a significant correlation between nitric oxide (NO(x)) concentrations and the sequential organ failure assessment (SOFA) score at day 2 (r = 0.598, p = 0.009), and ICU stay (r = 0.605, p = 0.006). Continuously high NO(x) levels correlated with organ failure. The pro-inflammatory mediators TNFα, IL-6 and NO(x), and also the Simplified Acute Physiology Score II (SAPS-II), discriminate survivors from non-survivors. According to logistic regression models, although these parameters are independently associated with the outcome, they do not improve the predictive power of the SAPS-II score for mortality risk.
CONCLUSIONS: Disturbances in inflammatory responses and increase in NO(x) generation seem to characterize early intra-abdominal sepsis, in which immune suppression is associated with an increased susceptibility to nosocomial infections. Sequential NO(x) determinations could be a useful approach for improving the management of patients with intra-abdominal sepsis.

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Year:  2010        PMID: 20976525     DOI: 10.1007/s00011-010-0267-4

Source DB:  PubMed          Journal:  Inflamm Res        ISSN: 1023-3830            Impact factor:   4.575


  31 in total

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2.  Role of nitric oxide in tolerance to lipopolysaccharide in mice.

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Review 7.  Therapeutic implications of immunoparalysis in critically ill patients.

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Review 8.  Immunoparalysis after multiple trauma.

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3.  Expression of genes belonging to the interacting TLR cascades, NADPH-oxidase and mitochondrial oxidative phosphorylation in septic patients.

Authors:  Laura A Nucci; Sidnéia S Santos; Milena K C Brunialti; Narendra Kumar Sharma; Flavia R Machado; Murillo Assunção; Luciano C P de Azevedo; Reinaldo Salomao
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4.  Metabolic systems analysis of LPS induced endothelial dysfunction applied to sepsis patient stratification.

Authors:  Sarah McGarrity; Ósk Anuforo; Haraldur Halldórsson; Andreas Bergmann; Skarphéðinn Halldórsson; Sirus Palsson; Hanne H Henriksen; Pär Ingemar Johansson; Óttar Rolfsson
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