| Literature DB >> 20967605 |
Hideo Matsumura1, Kentaro Yoshida, Shujun Luo, Detlev H Krüger, Günter Kahl, Gary P Schroth, Ryohei Terauchi.
Abstract
SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. Combined with the ultrahigh-throughput sequencing technologies, SuperSAGE enables analysis of millions of transcripts with lower cost and reduced effort and time. In this chapter, we present an updated protocol for this High-throughput SuperSAGE method with a special emphasis on the technique of library multiplexing.Mesh：
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Year: 2011 PMID： 20967605 DOI： 10.1007/978-1-60761-944-4_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745