BACKGROUND AND OBJECTIVES: Improvement of transfusion security in sub-Saharan countries requires the determination of priorities taking into account the specific context. PATIENTS AND METHODS: One hundred and forty patients with sickle cell disease (SCD) from one clinical centre for SCD in Kisangani, DRC were tested for HBsAg, anti-HIV antibodies, anti-HCV antibodies and for alloantibodies against red blood cells and human leucocyte antigens (HLA). RESULTS: Thirteen patients had not been transfused and were free of HBV, HIV or HCV infection. HBV, HIV and HCV infections were detected in 2/127 (1.6%), 1/127 (0.9%) and 10/127 (7.9%) transfused patients, respectively. All ten cases of HCV infection were associated with patients who had transfusions prior to the introduction of HCV testing in 2004 (P=0.043). Red blood cells and HLA alloantibodies were detected in 13/127 (10%) and 2/127 (1.6%), respectively. CONCLUSION: HCV testing should be a priority. The rhesus (Rh) phenotype, mainly the RhD antigen and the Kell antigen should be assessed in SCD patients. Further extended phenotyping and deleucocytation should not be considered as priorities.
BACKGROUND AND OBJECTIVES: Improvement of transfusion security in sub-Saharan countries requires the determination of priorities taking into account the specific context. PATIENTS AND METHODS: One hundred and forty patients with sickle cell disease (SCD) from one clinical centre for SCD in Kisangani, DRC were tested for HBsAg, anti-HIV antibodies, anti-HCV antibodies and for alloantibodies against red blood cells and human leucocyte antigens (HLA). RESULTS: Thirteen patients had not been transfused and were free of HBV, HIV or HCV infection. HBV, HIV and HCV infections were detected in 2/127 (1.6%), 1/127 (0.9%) and 10/127 (7.9%) transfused patients, respectively. All ten cases of HCV infection were associated with patients who had transfusions prior to the introduction of HCV testing in 2004 (P=0.043). Red blood cells and HLA alloantibodies were detected in 13/127 (10%) and 2/127 (1.6%), respectively. CONCLUSION: HCV testing should be a priority. The rhesus (Rh) phenotype, mainly the RhD antigen and the Kell antigen should be assessed in SCDpatients. Further extended phenotyping and deleucocytation should not be considered as priorities.
Authors: Paula F Blatyta; Shannon Kelly; Ester Sabino; Liliana Preiss; Franciane Mendes; Anna B Carneiro-Proietti; Daniela de Oliveira Werneck Rodrigues; Rosimere Mota; Paula Loureiro; Claudia Maximo; Miriam Park; Alfredo Mendrone-Jr; Thelma T Gonçalez; Cesar de Almeida Neto; Brian Custer Journal: Transfusion Date: 2019-12-05 Impact factor: 3.157
Authors: Sheila Makiala-Mandanda; Frédéric Le Gal; Nadine Ngwaka-Matsung; Steve Ahuka-Mundeke; Richard Onanga; Berthold Bivigou-Mboumba; Elisabeth Pukuta-Simbu; Athenaïs Gerber; Jessica L Abbate; Dieudonné Mwamba; Nicolas Berthet; Eric Maurice Leroy; Jean-Jacques Muyembe-Tamfum; Pierre Becquart Journal: J Clin Microbiol Date: 2017-02-15 Impact factor: 5.948
Authors: Shannon Kelly; Evan S Jacobs; Mars Stone; Sheila M Keating; Tzong-Hae Lee; Daniel Chafets; John Heitman; Melanie Dimapasoc; Eva Operskalski; Ward Hagar; Elliott Vichinsky; Michael P Busch; Philip J Norris; Brian Custer Journal: PLoS One Date: 2020-04-08 Impact factor: 3.240
Authors: P F Blatyta; S Kelly; T T Goncalez; A B Carneiro-Proietti; T Salomon; C Miranda; E Sabino; L Preiss; C Maximo; P Loureiro; B Custer; C de Almeida-Neto Journal: BMC Public Health Date: 2020-10-23 Impact factor: 3.295