Literature DB >> 20960144

Rubisco activase activity assays.

Csengele Barta1, A Elizabete Carmo-Silva, Michael E Salvucci.   

Abstract

Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) activase functions as a mechano-chemical motor protein using the energy from ATP hydrolysis to contort the structure of its target protein, Rubisco. This action modulates the activation state of Rubisco by removing tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites, thereby restoring the sites to catalytic competence. This chapter reports methods developed for assaying the two activities of Rubisco activase: ATP hydrolysis and Rubisco activation.

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Year:  2011        PMID： 20960144     DOI： 10.1007/978-1-60761-925-3_29

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Substitutions at the opening of the Rubisco central solvent channel affect holoenzyme stability and CO2/O 2 specificity but not activation by Rubisco activase.

Authors:  M Gloria Esquivel; Todor Genkov; Ana S Nogueira; Michael E Salvucci; Robert J Spreitzer
Journal:  Photosynth Res       Date:  2013-09-07       Impact factor: 3.573

2.  A single point mutation in the C-terminal extension of wheat Rubisco activase dramatically reduces ADP inhibition via enhanced ATP binding affinity.

Authors:  Andrew P Scafaro; David De Vleesschauwer; Nadine Bautsoens; Matthew A Hannah; Bart den Boer; Alexander Gallé; Jeroen Van Rie
Journal:  J Biol Chem       Date:  2019-09-17       Impact factor: 5.157

3.  Structure of green-type Rubisco activase from tobacco.

Authors:  Mathias Stotz; Oliver Mueller-Cajar; Susanne Ciniawsky; Petra Wendler; F Ulrich Hartl; Andreas Bracher; Manajit Hayer-Hartl
Journal:  Nat Struct Mol Biol       Date:  2011-11-06       Impact factor: 15.369

4.  The regulatory properties of Rubisco activase differ among species and affect photosynthetic induction during light transitions.

Authors:  A Elizabete Carmo-Silva; Michael E Salvucci
Journal:  Plant Physiol       Date:  2013-02-15       Impact factor: 8.340

5.  Activation of interspecies-hybrid Rubisco enzymes to assess different models for the Rubisco-Rubisco activase interaction.

Authors:  Rebekka M Wachter; Michael E Salvucci; A Elizabete Carmo-Silva; Csengele Barta; Todor Genkov; Robert J Spreitzer
Journal:  Photosynth Res       Date:  2013-04-24       Impact factor: 3.573

6.  Measuring Rubisco activity: challenges and opportunities of NADH-linked microtiter plate-based and 14C-based assays.

Authors:  Cristina R G Sales; Anabela Bernardes da Silva; Elizabete Carmo-Silva
Journal:  J Exp Bot       Date:  2020-09-19       Impact factor: 6.992

7.  Improved analysis of C4 and C3 photosynthesis via refined in vitro assays of their carbon fixation biochemistry.

Authors:  Robert E Sharwood; Balasaheb V Sonawane; Oula Ghannoum; Spencer M Whitney
Journal:  J Exp Bot       Date:  2016-04-27       Impact factor: 6.992
  7 in total

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