CONCLUSION: Our findings demonstrated that purple bamboo salt (PBS)-pharmaceutical acupuncture has an ameliorative effect on cisplatin-induced ototoxicity. OBJECTIVES: We have previously reported that PBS exhibited anti-allergic and anti-inflammatory actions in vitro and in vivo. Pharmaceutical acupuncture is a traditional oriental therapeutic technique that combines acupuncture with herbal treatment. The aim of this study was to investigate the protective effect and mechanism of PBS-pharmaceutical acupuncture against cisplatin-induced ototoxicity in the auditory cell line, HEI-OC1, and in vivo. METHODS: The ELISA method, a caspase-3 assay, an MTT assay, Western blot analysis, and a luciferase assay were utilized to investigate the effect of PBS in vivo and in vitro. RESULTS: When it was acupunctured at the Ermen acupoint (triple energizer meridian 21) after an administration of cisplatin, PBS-pharmaceutical acupuncture significantly suppressed interleukin (IL)-6 production and caspase-3 activation induced by cisplatin in the cochlea. In addition, PBS significantly inhibited cisplatin-induced apoptosis and IL-6 production in HEI-OC1 cells. PBS also suppressed cytochrome c release and caspase-3 activation, and it inhibited extracellular signal-related kinase and nuclear factor-κB activation in HEI-OC1 cells.
CONCLUSION: Our findings demonstrated that purple bamboo salt (PBS)-pharmaceutical acupuncture has an ameliorative effect on cisplatin-induced ototoxicity. OBJECTIVES: We have previously reported that PBS exhibited anti-allergic and anti-inflammatory actions in vitro and in vivo. Pharmaceutical acupuncture is a traditional oriental therapeutic technique that combines acupuncture with herbal treatment. The aim of this study was to investigate the protective effect and mechanism of PBS-pharmaceutical acupuncture against cisplatin-induced ototoxicity in the auditory cell line, HEI-OC1, and in vivo. METHODS: The ELISA method, a caspase-3 assay, an MTT assay, Western blot analysis, and a luciferase assay were utilized to investigate the effect of PBS in vivo and in vitro. RESULTS: When it was acupunctured at the Ermen acupoint (triple energizer meridian 21) after an administration of cisplatin, PBS-pharmaceutical acupuncture significantly suppressed interleukin (IL)-6 production and caspase-3 activation induced by cisplatin in the cochlea. In addition, PBS significantly inhibited cisplatin-induced apoptosis and IL-6 production in HEI-OC1 cells. PBS also suppressed cytochrome c release and caspase-3 activation, and it inhibited extracellular signal-related kinase and nuclear factor-κB activation in HEI-OC1 cells.
Authors: Z Altun; Y Olgun; P Ercetin; S Aktas; G Kirkim; B Serbetcioglu; N Olgun; E A Guneri Journal: Cell Prolif Date: 2013-11-29 Impact factor: 6.831