OBJECTIVE: The goal of this study is to examine if allogenic mesenchymal stem cell (MSC) transplantation is a useful therapy for left ischemic colon anastomosis in rats. Problems with anastomosis healing may lead to serious postoperative complications. Bone marrow-derived mesenchymal stem cells (BM-MSCs), which are also referred to as stromal progenitor cells, are self-renewing and expandable stem cells. Recent studies have suggested that BM-MSCs play a crucial role in the processes of intestinal repair and accelerate angiogenesis. METHODS: MSCs were isolated from rats before analysis by light and scanning electron microscopy. Forty male Wistar albino rats weighing 250-280 g were divided into four equal groups (n = 10) as follows: group 1: control, ischemic left colonic anastomoses (fourth day); group 2: control, ischemic left colonic anastomoses (seventh day); group 3: ischemic left colonic anastomoses + locally transplanted BM-MSCs (fourth day); group 4: ischemic left colonic anastomoses + locally transplanted BM-MSCs (seventh day). Histopathological features and anastomotic strength were evaluated. RESULTS: BM-MSCs therapy significantly accelerated all of the healing parameters for ischemic colonic anastomosis except for inflammation on the fourth day. On the seventh day, BM-MSCs augmented the levels of the hydroxyproline and bursting pressure. Histological parameters, especially angiogenesis, were also found to be important for healing of ischemic colonic anastomoses. CONCLUSIONS: This is the first study to use locally transplanted cell therapy for the healing of ischemic colonic anastomosis. BM-MSCs therapy significantly accelerated all of the healing parameters for ischemic colonic anastomosis.
OBJECTIVE: The goal of this study is to examine if allogenic mesenchymal stem cell (MSC) transplantation is a useful therapy for left ischemic colon anastomosis in rats. Problems with anastomosis healing may lead to serious postoperative complications. Bone marrow-derived mesenchymal stem cells (BM-MSCs), which are also referred to as stromal progenitor cells, are self-renewing and expandable stem cells. Recent studies have suggested that BM-MSCs play a crucial role in the processes of intestinal repair and accelerate angiogenesis. METHODS: MSCs were isolated from rats before analysis by light and scanning electron microscopy. Forty male Wistar albino rats weighing 250-280 g were divided into four equal groups (n = 10) as follows: group 1: control, ischemic left colonic anastomoses (fourth day); group 2: control, ischemic left colonic anastomoses (seventh day); group 3: ischemic left colonic anastomoses + locally transplanted BM-MSCs (fourth day); group 4: ischemic left colonic anastomoses + locally transplanted BM-MSCs (seventh day). Histopathological features and anastomotic strength were evaluated. RESULTS: BM-MSCs therapy significantly accelerated all of the healing parameters for ischemic colonic anastomosis except for inflammation on the fourth day. On the seventh day, BM-MSCs augmented the levels of the hydroxyproline and bursting pressure. Histological parameters, especially angiogenesis, were also found to be important for healing of ischemic colonic anastomoses. CONCLUSIONS: This is the first study to use locally transplanted cell therapy for the healing of ischemic colonic anastomosis. BM-MSCs therapy significantly accelerated all of the healing parameters for ischemic colonic anastomosis.
Authors: H I Friedman; H I F Friedman; M Fitzmaurice; J F Lefaivre; T Vecchiolla; D Clarke Journal: Plast Reconstr Surg Date: 2006-06 Impact factor: 4.730
Authors: E Donzelli; A Salvadè; P Mimo; M Viganò; M Morrone; R Papagna; F Carini; A Zaopo; M Miloso; M Baldoni; G Tredici Journal: Arch Oral Biol Date: 2006-10-16 Impact factor: 2.633
Authors: Samuel P Carmichael; Jaewook Shin; John W Vaughan; Prafulla K Chandra; John B Holcomb; Anthony J Atala Journal: J Surg Res Date: 2022-03-17 Impact factor: 2.417
Authors: Jong Han Yoo; Jae Ho Shin; Min Sung An; Tae Kwun Ha; Kwang Hee Kim; Ki Beom Bae; Tae Hyeon Kim; Chang Soo Choi; Kwan Hee Hong; Jeong Kim; Soo Jin Jung; Sun Hee Kim; Kuk Hwan Rho; Jong Tae Kim; Young Il Yang Journal: J Korean Soc Coloproctol Date: 2012-06-30
Authors: Sylwia Dabrowska; Andrea Del Fattore; Elzbieta Karnas; Malgorzata Frontczak-Baniewicz; Hanna Kozlowska; Maurizio Muraca; Miroslaw Janowski; Barbara Lukomska Journal: Int J Nanomedicine Date: 2018-03-19
Authors: Ahmed M Harraz; Guiting Lin; Lia Banie; Guifang Wang; Alan W Shindel; Yun-Ching Huang; Thomas M Fandel; Maurice Garcia; Tom F Lue; Ching-Shwun Lin Journal: Arab J Urol Date: 2011-08-06
Authors: Dirk Van de Putte; Christelle Demarquay; Elke Van Daele; Lara Moussa; Christian Vanhove; Marc Benderitter; Wim Ceelen; Piet Pattyn; Noëlle Mathieu Journal: Cell Transplant Date: 2017-12 Impact factor: 4.064