Carlos A Rubio1, Michael Owston, Abiel Orrego, Edward J Dick. 1. Gastrointestinal and Liver Pathology Research Laboratory, Department of Pathology, Karolinska Institute and University Hospital, 17176 Stockholm, Sweden. Carlos.Rubio@ki.se
Abstract
BACKGROUND: Gastric parietal cells in a baboon were recently found to be auto-fluorescent. AIM: To study gastric sections with a fluorescent microscope in a cohort of baboons. MATERIAL AND METHODS: Gastric sections from 38 baboons were stained with hematoxylin-eosin (H&E) and examined in a fluorescence microscope (FLM). The thickness of the parietal cell population was assessed at x 10 magnification. RESULTS: H&E stained all mucosal cells: fovelar, parietal and chief cells. When the same sections were analyzed with an FLM, only parietal cells were auto-fluorescent, whereas fovelar and chief cells remained non-fluorescent. Parietal cells formed a distinct, continuous auto-fluorescent band. The ratio of the auto-fluorescent parietal cell band/total mucosa ranged between 0.20 and 0.30. CONCLUSION: Gastric parietal cells became auto-fluorescent when H&E-stained sections from baboon stomachs were observed with an FLM. Eosin was the stain responsible for this optical phenomenon.
BACKGROUND: Gastric parietal cells in a baboon were recently found to be auto-fluorescent. AIM: To study gastric sections with a fluorescent microscope in a cohort of baboons. MATERIAL AND METHODS: Gastric sections from 38 baboons were stained with hematoxylin-eosin (H&E) and examined in a fluorescence microscope (FLM). The thickness of the parietal cell population was assessed at x 10 magnification. RESULTS:H&E stained all mucosal cells: fovelar, parietal and chief cells. When the same sections were analyzed with an FLM, only parietal cells were auto-fluorescent, whereas fovelar and chief cells remained non-fluorescent. Parietal cells formed a distinct, continuous auto-fluorescent band. The ratio of the auto-fluorescent parietal cell band/total mucosa ranged between 0.20 and 0.30. CONCLUSION: Gastric parietal cells became auto-fluorescent when H&E-stained sections from baboon stomachs were observed with an FLM. Eosin was the stain responsible for this optical phenomenon.
Authors: Valerie Legendre-Guillemin; Martina Metzler; Jean-Francois Lemaire; Jacynthe Philie; Lu Gan; Michael R Hayden; Peter S McPherson Journal: J Biol Chem Date: 2004-11-08 Impact factor: 5.157
Authors: Carlos A Rubio; Michael Owston; Abiel Orrego; Robert Nilsson; Hedwig Löfdahl; Gabriella Nesi; Edwards J Dick Journal: Anticancer Res Date: 2011-06 Impact factor: 2.480
Authors: Gregory T Walker; Guiyan Yang; Julia Y Tsai; Jorge L Rodriguez; Bevin C English; Franziska Faber; Lattha Souvannaseng; Brian P Butler; Renée M Tsolis Journal: Sci Adv Date: 2021-06-30 Impact factor: 14.136