| Literature DB >> 20951798 |
Jeena Santos-Ahmed1, Caitlin Brown, Stuart Duncan Smith, Paula Weston, Teresa Rasoulpour, Mary E Gilbert, Mary L Hixon.
Abstract
Exposure to 6-propyl-2-thio-uracil (PTU), a neonatal goitrogen, leads to increased testis size and sperm production in rodents. Akt1, a gene involved in cell survival and proliferation is also phosphorylated by thyroxine (T(4)). Therefore, we examined the requirement for Akt1 in germ cell survival following PTU-induced hypothyroidism. Experiments were performed using Akt1+/+, Akt1+/-, and Akt1-/- mice. PTU was administered (0.01% w/v) via the drinking water of dams from birth to PND21. At PND15, T(4) serum levels were similar in all control groups, and significantly lower in all exposed groups with a dramatic decrease in Akt1-/- mice. PTU-exposed Akt1-/- testes displayed smaller tubules, increased apoptosis, delayed lumen formation, and increased inhibin B and AMH mRNA. Relative adult testis weights were similar in all exposure groups; however, no increase in daily sperm production was observed in PTU-exposed Akt1-/- mice. In conclusion, Akt1 contributes to the effects of thyroid hormone on postnatal testis development.Entities:
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Year: 2010 PMID： 20951798 PMCID： PMC3033462 DOI： 10.1016/j.reprotox.2010.09.012
Source DB: PubMed Journal: Reprod Toxicol ISSN： 0890-6238 Impact factor: 3.143