Literature DB >> 20951745

A new method for detection of pandemic influenza virus using High Resolution Melting analysis of the neuraminidase gene.

David Varillas1, Jesus F Bermejo-Martin, Raquel Almansa, Silvia Rojo, Begoña Nogueira, Jose María Eiros, Lucia Rico, Verónica Iglesias, Raul Ortiz de Lejarazu.   

Abstract

Diagnostic methods based upon exclusive detection of haemagglutinin do not detect sequence variation in other gene segments of the Influenza A virus. A complementary approach is described based upon high-resolution melting curve analysis of the neuraminidase gene, an approach with the potential ability to detect small changes in the neuraminidase sequence without the need for specific probes.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID: 20951745     DOI: 10.1016/j.jviromet.2010.10.003

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  6 in total

1.  Characterization of influenza A(H1N1)pdm09 isolates of Peru using HRM, a post PCR molecular biology method.

Authors:  Priscila Lope; Huaringa Maribel; Mayta Egma; Bailon Henri; Padilla Carlos
Journal:  Bioinformation       Date:  2019-10-10

2.  Rapid detection of the H275Y oseltamivir resistance mutation in influenza A/H1N1 2009 by single base pair RT-PCR and high-resolution melting.

Authors:  Steven Y C Tong; Farshid Dakh; Aeron C Hurt; Yi-Mo Deng; Kevin Freeman; Peter K Fagan; Ian G Barr; Philip M Giffard
Journal:  PLoS One       Date:  2011-06-24       Impact factor: 3.240

3.  High resolution melting analysis: rapid and precise characterisation of recombinant influenza A genomes.

Authors:  Donata Kalthoff; Martin Beer; Bernd Hoffmann
Journal:  Virol J       Date:  2013-09-12       Impact factor: 4.099

4.  Nationwide surveillance of 18 respiratory viruses in patients with influenza-like illnesses: a pilot feasibility study in the French Sentinel Network.

Authors:  Alessandra Falchi; Clément Turbelin; Laurent Andreoletti; Christophe Arena; Thierry Blanchon; Isabelle Bonmarin; Thomas Hanslik; Marianne Leruez-Ville; Xavier De Lamballerie; Fabrice Carrat
Journal:  J Med Virol       Date:  2011-06-02       Impact factor: 2.327

5.  Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis.

Authors:  Faris Muhammad Gazali; Matin Nuhamunada; Rahma Nabilla; Endah Supriyati; Mohamad Saifudin Hakim; Eggi Arguni; Edwin Widyanto Daniwijaya; Titik Nuryastuti; Sofia Mubarika Haryana; Tri Wibawa; Nastiti Wijayanti
Journal:  Heliyon       Date:  2021-09-06

Review 6.  Molecular diagnosis of respiratory viruses.

Authors:  Lucy K Somerville; V Mala Ratnamohan; Dominic E Dwyer; Jen Kok
Journal:  Pathology       Date:  2015-04       Impact factor: 5.306

  6 in total

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