Literature DB >> 20951172

The small ribosomal protein S12P gene rpsL as an efficient positive selection marker in allelic exchange mutation systems for Corynebacterium glutamicum.

Il Kwon Kim1, Weol Kyu Jeong, Seong Han Lim, In Kwan Hwang, Young Ho Kim.   

Abstract

We report that the mutant rpsL K43R in streptomycin-resistant and lysine-producing Corynebacterium glutamicum is responsible for streptomycin resistance. In addition, we describe its effective application in gene modification in C. glutamicum.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20951172     DOI： 10.1016/j.mimet.2010.10.007

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  5 in total

1.  Abiotic stress resistance, a novel moonlighting function of ribosomal protein RPL44 in the halophilic fungus Aspergillus glaucus.

Authors:  Xiao-Dan Liu; Lixia Xie; Yi Wei; Xiaoyang Zhou; Baolei Jia; Jinliang Liu; Shihong Zhang
Journal:  Appl Environ Microbiol       Date:  2014-05-09       Impact factor: 4.792

2.  Homing endonuclease I-SceI-mediated Corynebacterium glutamicum ATCC 13032 genome engineering.

Authors:  Meng Wu; Yan Xu; Jun Yang; Guangdong Shang
Journal:  Appl Microbiol Biotechnol       Date:  2020-03-07       Impact factor: 4.813

3.  A suitable streptomycin-resistant mutant for constructing unmarked in-frame gene deletions using rpsL as a counter-selection marker.

Authors:  Yu-Kuo Tsai; Ci-Hong Liou; Jung-Chung Lin; Ling Ma; Chang-Phone Fung; Feng-Yee Chang; L Kristopher Siu
Journal:  PLoS One       Date:  2014-09-30       Impact factor: 3.240

4.  Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum.

Authors:  Jiao Liu; Yu Wang; Yujiao Lu; Ping Zheng; Jibin Sun; Yanhe Ma
Journal:  Microb Cell Fact       Date:  2017-11-16       Impact factor: 5.328

5.  A streptomycin resistance marker in H. parasuis based on site-directed mutations in rpsL gene to perform unmarked in-frame mutations and to verify natural transformation.

Authors:  Ke Dai; Xintian Wen; Yung-Fu Chang; Sanjie Cao; Qin Zhao; Xiaobo Huang; Rui Wu; Yong Huang; Qigui Yan; Xinfeng Han; Xiaoping Ma; Yiping Wen
Journal:  PeerJ       Date:  2018-01-11       Impact factor: 2.984
  5 in total

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