Literature DB >> 2094801

Histones H1 and H4 are present near the replication fork.

V Stefanovsky1, S Dimitrov, V Russanova, I Pashev.   

Abstract

The presence of histones H1 and H4 at the sites of actual DNA synthesis has been studied with Ehrlich ascites tumour cells, pulse labeled for different times with 3H-thymidine and then treated with formaldehyde to crosslink histones to DNA. The fixed chromatin fragments were sonicated to reduce the size of DNA, purified in a CsCl gradient and immunoprecipitated with antibodies to histones H1 and H4. Determination of specific radioactivity in precipitated probes showed that both histones have been associated with nascent DNA even upon 1 min pulse with 3H-thymidine, thus indicating their presence near the replication fork.

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Year:  1990        PMID： 2094801     DOI： 10.1007/bf00429890

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  15 in total

1.  Assembly of newly replicated chromatin.

Authors:  A Worcel; S Han; M L Wong
Journal:  Cell       Date:  1978-11       Impact factor: 41.582

2.  Assembly of SV40 and polyoma minichromosomes during replication.

Authors:  C Crémisi; A Chestier; M Yaniv
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

3.  Electron microscopic analysis of chromosome metabolism in the Drosophila melanogaster embryo.

Authors:  S L McKnight; M Bustin; O L Miller
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1978

4.  The globular region of histone H5 is equally accessible to antibodies in relaxed and condensed chromatin.

Authors:  A Thibodeau; A Ruiz-Carrillo
Journal:  J Biol Chem       Date:  1988-11-05       Impact factor: 5.157

5.  Accessibility of the globular domain of histones H1 and H5 to antibodies upon folding of chromatin.

Authors:  V R Russanova; S I Dimitrov; V L Makarov; I G Pashev
Journal:  Eur J Biochem       Date:  1987-09-01

6.  Protein-DNA crosslinking in reconstituted nucleohistone, nuclei and whole cells by picosecond UV laser irradiation.

Authors:  D Angelov; S I Dimitrov; V R Russanova; E Keskinova; I G Pashev
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971

7.  Mapping protein-DNA interactions in vivo with formaldehyde: evidence that histone H4 is retained on a highly transcribed gene.

Authors:  M J Solomon; P L Larsen; A Varshavsky
Journal:  Cell       Date:  1988-06-17       Impact factor: 41.582

8.  Conservative assembly and segregation of nucleosomal histones.

Authors:  I M Leffak; R Grainger; H Weintraub
Journal:  Cell       Date:  1977-11       Impact factor: 41.582

9.  Histone synthesis and deposition in the G1 and S phases of hepatoma tissue culture cells.

Authors:  V Jackson; R Chalkley
Journal:  Biochemistry       Date:  1985-11-19       Impact factor: 3.162

10.  Structure of chromatin at deoxyribonucleic acid replication forks: location of the first nucleosomes on newly synthesized simian virus 40 deoxyribonucleic acid.

Authors:  T M Herman; M L DePamphilis; P M Wassarman
Journal:  Biochemistry       Date:  1981-02-03       Impact factor: 3.162
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  1 in total

1.  Histone H1 deposition and histone-DNA interactions in replicating chromatin.

Authors:  S Bavykin; L Srebreva; T Banchev; R Tsanev; J Zlatanova; A Mirzabekov
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205
  1 in total

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