Literature DB >> 20945296

Measurement of aryl and alcohol sulfotransferase activity.

J J Sheng1, V Sharma, M W Duffel.   

Abstract

Two methods are presented for determining the catalytic activity of aryl and alcohol sulfotransferases. The first assay is a simple and rapid procedure that is based on the extraction of a paired ion formed between the product organic sulfate and methylene blue. The second method employs HPLC analysis of the substrate-dependent formation of PAP, an assay that is particularly useful when the sulfuric acid ester product is chemically unstable.

Entities:  

Mesh：

Substances：

Year:  2001        PMID： 20945296     DOI： 10.1002/0471140856.tx0405s08

Source DB:  PubMed          Journal:  Curr Protoc Toxicol        ISSN： 1934-9254


  4 in total

1.  Hydroxylated and sulfated metabolites of commonly occurring airborne polychlorinated biphenyls inhibit human steroid sulfotransferases SULT1E1 and SULT2A1.

Authors:  Victoria S Parker; Edwin J Squirewell; Hans-Joachim Lehmler; Larry W Robertson; Michael W Duffel
Journal:  Environ Toxicol Pharmacol       Date:  2018-02-03       Impact factor: 4.860

2.  Mechanistic insights into the specificity of human cytosolic sulfotransferase 2A1 (hSULT2A1) for hydroxylated polychlorinated biphenyls through the use of fluoro-tagged probes.

Authors:  E J Ekuase; T J van 't Erve; A Rahaman; L W Robertson; M W Duffel; G Luthe
Journal:  Environ Sci Pollut Res Int       Date:  2015-07-14       Impact factor: 4.223

3.  Detection and Quantification of Polychlorinated Biphenyl Sulfates in Human Serum.

Authors:  Duo Zhang; Panithi Saktrakulkla; Kristopher Tuttle; Rachel F Marek; Hans-Joachim Lehmler; Kai Wang; Keri C Hornbuckle; Michael W Duffel
Journal:  Environ Sci Technol       Date:  2021-01-27       Impact factor: 9.028

4.  Sulfated metabolites of polychlorinated biphenyls are high-affinity ligands for the thyroid hormone transport protein transthyretin.

Authors:  Fabian A Grimm; Hans-Joachim Lehmler; Xianran He; Larry W Robertson; Michael W Duffel
Journal:  Environ Health Perspect       Date:  2013-04-12       Impact factor: 9.031
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.