OBJECTIVE: To estimate the prevalence of HIV-1 subtypes and analyze factors associated. METHODS: A cross-sectional study was performed with a convenience sample of 80 adult HIV-positive patients, users of an AIDS/STD specialized service, in the city of Canoas, Southern Brazil, between July 2008 and January 2009. Determination of HIV subtypes was performed with the amplification of viral genome fragment, using polymerase chain reaction, followed by sequencing of the amplified fragments. Sociodemographic, clinical and behavioral variables were collected in a structured questionnaire. Univariate statistical analysis was performed, using chi-square test and Student's t-test. RESULTS: A higher prevalence of subtype C was found (43.8%; 95% CI: 32.9;54.6), followed by CRF31_BC (35.0%; 95% CI: 24.6;45.5) and subtypes B (18.8%; 95% CI: 10.2;27.3) and F (2.4%; 95% CI: 0;5.9). Other HIV-1 subtypes were not observed. Patients infected with CRF31_BC were diagnosed more recently than patients infected with subtype B (p<0.05). In addition, there was a higher frequency of co-infection with other viruses (hepatitis B and C and human T-lymphotropic viruses) in individuals with CRF31_BC, compared to other subtypes. With regard to sociodemographic aspects, there were no differences in the distribution of subtypes and recombinant forms, in terms of gender and sexual practices. CONCLUSIONS: Results obtained indicate a higher frequency of subtype C and CRF31_BC in this urban center of Southern Brazil, with possible different ways of transmission.
OBJECTIVE: To estimate the prevalence of HIV-1 subtypes and analyze factors associated. METHODS: A cross-sectional study was performed with a convenience sample of 80 adult HIV-positive patients, users of an AIDS/STD specialized service, in the city of Canoas, Southern Brazil, between July 2008 and January 2009. Determination of HIV subtypes was performed with the amplification of viral genome fragment, using polymerase chain reaction, followed by sequencing of the amplified fragments. Sociodemographic, clinical and behavioral variables were collected in a structured questionnaire. Univariate statistical analysis was performed, using chi-square test and Student's t-test. RESULTS: A higher prevalence of subtype C was found (43.8%; 95% CI: 32.9;54.6), followed by CRF31_BC (35.0%; 95% CI: 24.6;45.5) and subtypes B (18.8%; 95% CI: 10.2;27.3) and F (2.4%; 95% CI: 0;5.9). Other HIV-1 subtypes were not observed. Patients infected with CRF31_BC were diagnosed more recently than patients infected with subtype B (p<0.05). In addition, there was a higher frequency of co-infection with other viruses (hepatitis B and C and human T-lymphotropic viruses) in individuals with CRF31_BC, compared to other subtypes. With regard to sociodemographic aspects, there were no differences in the distribution of subtypes and recombinant forms, in terms of gender and sexual practices. CONCLUSIONS: Results obtained indicate a higher frequency of subtype C and CRF31_BC in this urban center of Southern Brazil, with possible different ways of transmission.
Authors: Sabrina Em Almeida; Rubia M de Medeiros; Dennis M Junqueira; Tiago Gräf; Caroline P B Passaes; Gonzalo Bello; Mariza G Morgado; Monick L Guimarães Journal: Virol J Date: 2012-12-12 Impact factor: 4.099
Authors: Gonzalo Bello; Paolo M de A Zanotto; Atila Iamarino; Tiago Gräf; Aguinaldo R Pinto; José C Couto-Fernandez; Mariza G Morgado Journal: PLoS One Date: 2012-04-18 Impact factor: 3.240
Authors: Gabriel de Deus Vieira; Ana Raquel Paz Dos Reis; Francisco Ormidiel Teles de Alcântara Augusto; Karina Reis Martins; Paulo Roberto Fernandes Kern; Thairini Fuza de Souza; Sérgio de Almeida Basano; Luís Marcelo Aranha Camargo; Camila Maciel de Sousa Journal: Infect Dis Poverty Date: 2015-07-11 Impact factor: 4.520