| Literature DB >> 20938844 |
Ann Williams1, D Chas Mangham.
Abstract
This chapter outlines the methodology for the detection of mesenchymal-tumour-specific translocations in formalin-fixed paraffin-embedded tissue (FFPET) using the reverse transcriptase-polymerase chain reaction (RT-PCR).It includes the design of appropriate primer pairs and the necessary pretreatment of the FFPET sections to give the maximum yield of analyzable RNA, in terms of both quantity and quality.Entities:
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Year: 2011 PMID： 20938844 DOI： 10.1007/978-1-60761-947-5_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745