| Literature DB >> 20938729 |
Hongying Zheng1, Fei Yan, Yuwen Lu, Liying Sun, Lin Lin, Li Cai, Mingsheng Hou, Jianping Chen.
Abstract
The helper component-proteinase (HC-Pro) of potyviruses is a multifunctional protein involved in aphid transmission, polyprotein processing, cell-to-cell and long-distance movement, genome amplification and symptom expression. The HC-Pros of several potyviruses interact with themselves but the key domains responsible for self-interaction are apparently not conserved. In our experiments, yeast two-hybrid assays and bimolecular fluorescence complementation showed that Turnip mosaic virus (TuMV) HC-Pro interacted with itself in yeast cells, plant cells and insect cells. It was also shown that the central and C-terminal regions of the HC-Pro participated in these self-interactions. Fluorescence microscopy showed that TuMV HC-Pro was present in the cytoplasm and formed aggregates along the ER.Entities:
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Year: 2010 PMID： 20938729 DOI： 10.1007/s11262-010-0538-8
Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.198