BACKGROUND: We have isolated mesenchymal stem cells (MSCs) from tracheal aspirates of premature infants with respiratory distress. Under the influence of transforming growth factor β, MSCs differentiate into α-smooth-muscle actin-expressing myofibroblasts. Myofibroblasts are increased in the lungs of patients with bronchopulmonary dysplasia (BPD), a chronic lung disease of prematurely born infants. OBJECTIVE: We tested whether isolation of MSCs from tracheal aspirates of premature infants with respiratory distress during the first week of life correlates with BPD. METHODS: Eighty-four infants born at a gestational age of <33 weeks and requiring mechanical ventilation were studied. Aspirates were collected during suctioning and centrifuged. Cell pellets were resuspended in culture medium and plated. Adherent cells were grown to confluence. RESULTS: MSCs were isolated from the tracheal aspirates of 56 infants; 28 aspirate samples showed no MSCs. There was no statistical difference in gestational age or birth weight between the MSC and no-MSC groups. In the MSC group, 12 infants died and 25 developed BPD, as defined by a requirement for supplemental oxygen at 36 weeks' postmenstrual age. In the no-MSC group, 6 infants died and 1 developed BPD. Accounting for potential influences of gender, birth weight, gestational age, number of tracheal aspirate samples taken, and the duration of endotracheal intubation (up to 7 days), isolation of MSCs increased the adjusted odds ratio of BPD more than 21-fold (95% confidence interval: 1.82-265.85). CONCLUSIONS: Isolation of tracheal aspirate MSCs predicts the development of BPD, which suggests that MSCs play an important role in the pathogenesis of this disease.
BACKGROUND: We have isolated mesenchymal stem cells (MSCs) from tracheal aspirates of premature infants with respiratory distress. Under the influence of transforming growth factor β, MSCs differentiate into α-smooth-muscle actin-expressing myofibroblasts. Myofibroblasts are increased in the lungs of patients with bronchopulmonary dysplasia (BPD), a chronic lung disease of prematurely born infants. OBJECTIVE: We tested whether isolation of MSCs from tracheal aspirates of premature infants with respiratory distress during the first week of life correlates with BPD. METHODS: Eighty-four infants born at a gestational age of <33 weeks and requiring mechanical ventilation were studied. Aspirates were collected during suctioning and centrifuged. Cell pellets were resuspended in culture medium and plated. Adherent cells were grown to confluence. RESULTS: MSCs were isolated from the tracheal aspirates of 56 infants; 28 aspirate samples showed no MSCs. There was no statistical difference in gestational age or birth weight between the MSC and no-MSC groups. In the MSC group, 12 infants died and 25 developed BPD, as defined by a requirement for supplemental oxygen at 36 weeks' postmenstrual age. In the no-MSC group, 6 infants died and 1 developed BPD. Accounting for potential influences of gender, birth weight, gestational age, number of tracheal aspirate samples taken, and the duration of endotracheal intubation (up to 7 days), isolation of MSCs increased the adjusted odds ratio of BPD more than 21-fold (95% confidence interval: 1.82-265.85). CONCLUSIONS: Isolation of tracheal aspirate MSCs predicts the development of BPD, which suggests that MSCs play an important role in the pathogenesis of this disease.
Authors: Thomas K Shimotake; Farzana M Izhar; Kandelaria Rumilla; Jing Li; Alan Tan; Kristen Page; Allan R Brasier; Michael D Schreiber; Marc B Hershenson Journal: Pediatr Res Date: 2004-10-20 Impact factor: 3.756
Authors: Alfin G Vicencio; Chun Geun Lee; Soo Jung Cho; Oliver Eickelberg; Ying Chuu; Gabriel G Haddad; Jack A Elias Journal: Am J Respir Cell Mol Biol Date: 2004-08-27 Impact factor: 6.914
Authors: P Lindahl; L Karlsson; M Hellström; S Gebre-Medhin; K Willetts; J K Heath; C Betsholtz Journal: Development Date: 1997-10 Impact factor: 6.868
Authors: Antonia P Popova; J Kelley Bentley; Anuli C Anyanwu; Michelle N Richardson; Marisa J Linn; Jing Lei; Elizabeth J Wong; Adam M Goldsmith; Gloria S Pryhuber; Marc B Hershenson Journal: Am J Physiol Lung Cell Mol Physiol Date: 2012-07-06 Impact factor: 5.464
Authors: Darcy E Wagner; Wellington V Cardoso; Sarah E Gilpin; Susan Majka; Harald Ott; Scott H Randell; Bernard Thébaud; Thomas Waddell; Daniel J Weiss Journal: Ann Am Thorac Soc Date: 2016-08
Authors: Piero Anversa; Mark A Perrella; Stella Kourembanas; Augustine M K Choi; Joseph Loscalzo Journal: Eur J Clin Invest Date: 2012-03-21 Impact factor: 4.686
Authors: Paul D Bozyk; Antonia P Popova; John Kelley Bentley; Adam M Goldsmith; Marisa J Linn; Daniel J Weiss; Marc B Hershenson Journal: Stem Cells Dev Date: 2011-04-06 Impact factor: 3.272
Authors: Tarek L Mohamed; Hang T Nguyen; Amal Abdul-Hafez; Vinh X Dang; MyTrang T Dang; Ira H Gewolb; Bruce D Uhal Journal: Exp Lung Res Date: 2016-04 Impact factor: 2.459