CONTEXT: Previously, we demonstrated that CD11b is expressed on peripheral blood memory B cells, and it plays an important role in the migration of B cells. And epigallocatechin gallate (EGCG), a bioactive component of green tea, by binding to CD11b, expressed on CD8(+) cytotoxic T cells, inhibited their migratory ability, one possible mechanism of the antiallergic activity of EGCG. OBJECTIVE: Here, we investigated whether EGCG also affected CD11b expressed on B cells, similar to cytotoxic T cells. MATERIALS AND METHODS: Isolated peripheral blood CD19(+) B cells were treated with EGCG and the change in the expression of CD11b was analyzed using flow cytometry. The effects of EGCG on the ability of B cells to adhere to and to transmigrate through the endothelial cell layer were evaluated using the transwell assay. RESULTS: EGCG significantly suppressed the apparent expression of CD11b on B cells, in the flow-cytometric analysis, and this apparent suppression was speculated to be dependent on the competitive binding of EGCG to CD11b. EGCG also significantly suppressed CD11b-mediated migration and adhesion of B cells to endothelial cells. DISCUSSION AND CONCLUSION: EGCG has a strong suppressive activity on the adhesive and migratory abilities of peripheral blood B cells. This suppressive activity was mediated by the binding of EGCG to CD11b on B cells, and the consequent suppression of B-cell extravasation to the extravascular space. Because B cell plays an important role in the humoral immunity, EGCG could be a promising drug for the prevention and/or treatment of allergic and/or autoimmune diseases.
CONTEXT: Previously, we demonstrated that CD11b is expressed on peripheral blood memory B cells, and it plays an important role in the migration of B cells. And epigallocatechin gallate (EGCG), a bioactive component of green tea, by binding to CD11b, expressed on CD8(+) cytotoxic T cells, inhibited their migratory ability, one possible mechanism of the antiallergic activity of EGCG. OBJECTIVE: Here, we investigated whether EGCG also affected CD11b expressed on B cells, similar to cytotoxic T cells. MATERIALS AND METHODS: Isolated peripheral blood CD19(+) B cells were treated with EGCG and the change in the expression of CD11b was analyzed using flow cytometry. The effects of EGCG on the ability of B cells to adhere to and to transmigrate through the endothelial cell layer were evaluated using the transwell assay. RESULTS:EGCG significantly suppressed the apparent expression of CD11b on B cells, in the flow-cytometric analysis, and this apparent suppression was speculated to be dependent on the competitive binding of EGCG to CD11b. EGCG also significantly suppressed CD11b-mediated migration and adhesion of B cells to endothelial cells. DISCUSSION AND CONCLUSION:EGCG has a strong suppressive activity on the adhesive and migratory abilities of peripheral blood B cells. This suppressive activity was mediated by the binding of EGCG to CD11b on B cells, and the consequent suppression of B-cell extravasation to the extravascular space. Because B cell plays an important role in the humoral immunity, EGCG could be a promising drug for the prevention and/or treatment of allergic and/or autoimmune diseases.
Authors: Ahmad Jalloh; Antwoine Flowers; Charles Hudson; Dale Chaput; Jennifer Guergues; Stanley M Stevens; Paula C Bickford Journal: Int J Mol Sci Date: 2021-06-14 Impact factor: 5.923
Authors: Miguel Sánchez de Santiago; José Luis González Arribas; Yolanda Moral Llamas; Iveta Becvarova; Hein Meyer Journal: BMC Vet Res Date: 2021-11-19 Impact factor: 2.741
Authors: Claudia Urueña; Ricardo Ballesteros-Ramírez; Alejandra Gomez-Cadena; Alfonso Barreto; Karol Prieto; Sandra Quijano; Pablo Aschner; Carlos Martínez; Maria I Zapata-Cardona; Hajar El-Ahanidi; Camilla Jandus; Lizdany Florez-Alvarez; Maria Teresa Rugeles; Wildeman Zapata-Builes; Angel Alberto Garcia; Susana Fiorentino Journal: Front Med (Lausanne) Date: 2022-09-08