| Literature DB >> 20933506 |
Junfeng Wang1, Kangcheng Ruan.
Abstract
The expression of 350 miRNAs during the rat epididymal development was profiled by a home-made miRNA microarray recently. The results showed that the expression of miR-335 decreased from postnatal day 7 to 49 in the rat epididymis. Few studies on miR-335's roles in the epididymis and its targets have been reported so far. The bioinformatic analysis indicated that one of miR-335's putative targets is RASA1, a known gene related to cell proliferation and anti-apoptosis, which may contribute to the epididymal development. It was found that the overexpression of miR-335 in NIH/3T3 cells caused the suppression of RASA1 expression. The result of luciferase targeting assay confirmed that the mRNA of RASA1 was targeted by miR-335. In addition, it was also found that the temporal expression of RASA1 was inverse to that of miR-335 during the epididymal development in the rat. These results indicated that the reduction of miR-335 at least in part caused the upregulation of RASA1 in the rat epididymis. Therefore, miR-335 can be involved in the epididymal development by affecting the expression of RASA1.Entities:
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Year: 2010 PMID： 20933506 DOI： 10.1016/j.bbrc.2010.10.002
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575