Literature DB >> 20926700

Development, characterization, and application of monoclonal antibodies against severe acute respiratory syndrome coronavirus nucleocapsid protein.

Dipankar Das1, Sriram Kammila, Mavanur R Suresh.   

Abstract

Five monoclonal antibodies (MAbs) against recombinant nucleocapsid protein (NP) of severe acute respiratory syndrome (SARS)-causing coronavirus (CoV) were developed by hybridoma technology. Epitope mapping by Western blotting showed that these anti-SARS-CoV NP MAbs bind to distinct domains of NP. These anti-SARS-CoV NP MAbs, with their high specificity, are potentially ideal candidates for developing early and sensitive diagnostic assays for SARS-CoV.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20926700      PMCID： PMC3008203          DOI： 10.1128/CVI.00293-10

Source DB:  PubMed          Journal:  Clin Vaccine Immunol        ISSN： 1556-679X


  14 in total

1.  Monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay reveals high sensitivity of the nucleocapsid protein in acute-phase sera of severe acute respiratory syndrome patients.

Authors:  Biao Di; Wei Hao; Yang Gao; Ming Wang; Ya-di Wang; Li-wen Qiu; Kun Wen; Duan-hua Zhou; Xin-wei Wu; En-jie Lu; Zhi-yong Liao; Ya-bo Mei; Bo-jian Zheng; Xiao-yan Che
Journal:  Clin Diagn Lab Immunol       Date:  2005-01

2.  Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay.

Authors:  Susanna K P Lau; Patrick C Y Woo; Beatrice H L Wong; Hoi-Wah Tsoi; Gibson K S Woo; Rosana W S Poon; Kwok-Hung Chan; William I Wei; J S Malik Peiris; Kwok-Yung Yuen
Journal:  J Clin Microbiol       Date:  2004-07       Impact factor: 5.948

3.  Molecular targets for diagnostics and therapeutics of severe acute respiratory syndrome (SARS-CoV).

Authors:  Mavanur R Suresh; Pravin K Bhatnagar; Dipankar Das
Journal:  J Pharm Pharm Sci       Date:  2008-04-19       Impact factor: 2.327

4.  Production and characterization of monoclonal antibodies against different epitopes of Ebola virus antigens.

Authors:  Soraya Shahhosseini; Dipankar Das; Xiangguo Qiu; Heinz Feldmann; Steven M Jones; Mavanur R Suresh
Journal:  J Virol Methods       Date:  2007-03-19       Impact factor: 2.014

5.  Nucleocapsid protein as early diagnostic marker for SARS.

Authors:  Xiao-Yan Che; Wei Hao; Yadi Wang; Biao Di; Kai Yin; Yin-Chao Xu; Chang-Sen Feng; Zhuo-Yue Wan; Vincent C C Cheng; Kwok-Yung Yuen
Journal:  Emerg Infect Dis       Date:  2004-11       Impact factor: 6.883

6.  Copious production of SARS-CoV nucleocapsid protein employing codon optimized synthetic gene.

Authors:  Dipankar Das; Mavanur R Suresh
Journal:  J Virol Methods       Date:  2006-08-09       Impact factor: 2.014

7.  A rapid point of care immunoswab assay for SARS-CoV detection.

Authors:  Sriram Kammila; Dipankar Das; Pravin K Bhatnagar; Hoon H Sunwoo; Gustavo Zayas-Zamora; Malcolm King; Mavanur R Suresh
Journal:  J Virol Methods       Date:  2008-07-11       Impact factor: 2.014

Review 8.  The spike protein of SARS-CoV--a target for vaccine and therapeutic development.

Authors:  Lanying Du; Yuxian He; Yusen Zhou; Shuwen Liu; Bo-Jian Zheng; Shibo Jiang
Journal:  Nat Rev Microbiol       Date:  2009-02-09       Impact factor: 60.633

9.  Sequential affinity purification of peroxidase tagged bispecific anti-SARS-CoV antibodies on phenylboronic acid agarose.

Authors:  Pravin K Bhatnagar; Dipankar Das; Mavanur R Suresh
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2008-01-17       Impact factor: 3.205

Review 10.  SARS: clinical virology and pathogenesis.

Authors:  John Nicholls; Xiao-Ping Dong; Gu Jiang; Malik Peiris
Journal:  Respirology       Date:  2003-11       Impact factor: 6.424
View more
  2 in total

1.  Development of a SARS-CoV-2-specific biosensor for antigen detection using scFv-Fc fusion proteins.

Authors:  Hye-Yeon Kim; Jong-Hwan Lee; Mi Jeong Kim; Sun Cheol Park; Minsuk Choi; Wonbin Lee; Keun Bon Ku; Bum Tae Kim; Edmond Changkyun Park; Hong Gi Kim; Seung Il Kim
Journal:  Biosens Bioelectron       Date:  2020-11-30       Impact factor: 10.618

2.  Immunoreactivity characterisation of the three structural regions of the human coronavirus OC43 nucleocapsid protein by Western blot: implications for the diagnosis of coronavirus infection.

Authors:  Fang-Ying Liang; Leng-Chieh Lin; Tsung-Ho Ying; Chen-Wen Yao; Tswen-Kei Tang; Yi-Wen Chen; Ming-Hon Hou
Journal:  J Virol Methods       Date:  2012-11-19       Impact factor: 2.014
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.