Literature DB >> 2091691

Current methods for YAC clone characterization.

D L Nelson1.   

Abstract

Yeast artificial chromosome (YAC) cloning has allowed isolation of much longer DNA fragments than was previously possible. While this technology makes feasible the isolation of large regions of complex genomes in overlapping cloned segments, it has also required altered methods for manipulation of cloned DNAs. In particular, the isolation of insert sequences from YAC clones has been especially difficult. Methods for characterization of YAC inserts are described and compared.

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Year:  1990        PMID： 2091691     DOI： 10.1016/0735-0651(90)90014-7

Source DB:  PubMed          Journal:  Genet Anal Tech Appl        ISSN： 1050-3862


  3 in total

1.  Rescue of end fragments of yeast artificial chromosomes by homologous recombination in yeast.

Authors:  G G Hermanson; M F Hoekstra; D L McElligott; G A Evans
Journal:  Nucleic Acids Res       Date:  1991-09-25       Impact factor: 16.971

2.  von Hippel-Lindau disease: identification of deletion mutations by pulsed-field gel electrophoresis.

Authors:  M Yao; F Latif; M L Orcutt; I Kuzmin; T Stackhouse; F W Zhou; K Tory; F M Duh; F Richards; E Maher
Journal:  Hum Genet       Date:  1993-12       Impact factor: 4.132

3.  Assignment of the gene responsible for cystinuria (rBAT) and of markers D2S119 and D2S177 to 2p16 by fluorescence in situ hybridization.

Authors:  M J Calonge; M Nadal; S Calvano; X Testar; L Zelante; A Zorzano; X Estivill; P Gasparini; M Palacín; V Nunes
Journal:  Hum Genet       Date:  1995-06       Impact factor: 4.132
  3 in total

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