Literature DB >> 20890902

Serine/threonine protein phosphatase assays.

Thomas McAvoy1, Angus C Nairn.   

Abstract

Methods for assaying serine/threonine protein phosphatases are discussed. Three commonly used protocols are presented that employ either colorimetric or radiometric assays. These methods can be used to assay a variety of preparations of serine/threonine phosphatases, from crude lysates to purified proteins. Strategies for the application of a particular protocol for a particular purpose are discussed. The assays can be used in either a high-throughput mode where simple comparison of activities can be done, or in specific assays where kinetic data can be derived.

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Year:  2010        PMID： 20890902      PMCID： PMC2965627          DOI： 10.1002/0471142727.mb1818s92

Source DB:  PubMed          Journal:  Curr Protoc Mol Biol        ISSN： 1934-3647


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Authors:  Mark Swingle; Li Ni; Richard E Honkanen
Journal:  Methods Mol Biol       Date:  2007

Review 6.  From promiscuity to precision: protein phosphatases get a makeover.

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Authors:  S D Killilea; Q Cheng; Z X Wang
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5.  SIV-Mediated Synaptic Dysfunction Is Associated with an Increase in Synapsin Site 1 Phosphorylation and Impaired PP2A Activity.

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10.  Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman.

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