Literature DB >> 20889788

Participation of valine 171 in alpha-Helix 5 of Bacillus thuringiensis Cry1Ab delta-endotoxin in translocation of toxin into Lymantria dispar midgut membranes.

Oscar Alzate1, Cristina Osorio, Alvaro M Florez, Donald H Dean.   

Abstract

The Cry1Ab δ-endotoxin V171C mutant protein exhibits a 25-fold increase in toxicity against Lymantria dispar, which correlates with a faster rate of partitioning into the midgut membrane and slightly decreased protein stability. This is an insect-specific mechanism; similar results were not observed in Manduca sexta, another Cry1Ab δ-endotoxin-susceptible insect.

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Year:  2010        PMID： 20889788      PMCID： PMC2988598          DOI： 10.1128/AEM.01428-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Effects of disulfide bridges in domain I of Bacillus thuringiensis Cry1Aa delta-endotoxin on ion-channel formation in biological membranes.

Authors:  O Alzate; T You; M Claybon; C Osorio; A Curtiss; D H Dean
Journal:  Biochemistry       Date:  2006-11-14       Impact factor: 3.162

2.  Role of alpha-helix seven of Bacillus thuringiensis Cry1Ab delta-endotoxin in membrane insertion, structural stability, and ion channel activity.

Authors:  E P Alcantara; O Alzate; M K Lee; A Curtiss; D H Dean
Journal:  Biochemistry       Date:  2001-02-27       Impact factor: 3.162

3.  Redesigning Bacillus thuringiensis Cry1Aa toxin into a mosquito toxin.

Authors:  Xinyan Sylvia Liu; Donald H Dean
Journal:  Protein Eng Des Sel       Date:  2006-01-25       Impact factor: 1.650

4.  Production of chymotrypsin-resistant Bacillus thuringiensis Cry2Aa1 delta-endotoxin by protein engineering.

Authors:  M Audtho; A P Valaitis; O Alzate; D H Dean
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

5.  Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation.

Authors:  P Grochulski; L Masson; S Borisova; M Pusztai-Carey; J L Schwartz; R Brousseau; M Cygler
Journal:  J Mol Biol       Date:  1995-12-01       Impact factor: 5.469

6.  Cysteine-scanning mutagenesis of transmembrane domain XII and the flanking periplasmic loop in the lactose permease of EScherichia coli.

Authors:  M M He; J Sun; H R Kaback
Journal:  Biochemistry       Date:  1996-10-01       Impact factor: 3.162

7.  Irreversible binding kinetics of Bacillus thuringiensis CryIA delta-endotoxins to gypsy moth brush border membrane vesicles is directly correlated to toxicity.

Authors:  Y Liang; S S Patel; D H Dean
Journal:  J Biol Chem       Date:  1995-10-20       Impact factor: 5.157

8.  Role of domain II, loop 2 residues of Bacillus thuringiensis CryIAb delta-endotoxin in reversible and irreversible binding to Manduca sexta and Heliothis virescens.

Authors:  F Rajamohan; J A Cotrill; F Gould; D H Dean
Journal:  J Biol Chem       Date:  1996-02-02       Impact factor: 5.157

9.  Effect of insect larval midgut proteases on the activity of Bacillus thuringiensis Cry toxins.

Authors:  Mélanie Fortier; Vincent Vachon; Roger Frutos; Jean-Louis Schwartz; Raynald Laprade
Journal:  Appl Environ Microbiol       Date:  2007-08-10       Impact factor: 4.792

10.  Ser170 of Bacillus thuringiensis Cry1Ab delta-endotoxin becomes anchored in a hydrophobic moiety upon insertion of this protein into Manduca sexta brush border membranes.

Authors:  Oscar Alzate; Craig F Hemann; Cristina Osorio; Russ Hille; Donald H Dean
Journal:  BMC Biochem       Date:  2009-10-19       Impact factor: 4.059
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  5 in total

Review 1.  Evolution of Bacillus thuringiensis Cry toxins insecticidal activity.

Authors:  Alejandra Bravo; Isabel Gómez; Helena Porta; Blanca Ines García-Gómez; Claudia Rodriguez-Almazan; Liliana Pardo; Mario Soberón
Journal:  Microb Biotechnol       Date:  2012-03-29       Impact factor: 5.813

Review 2.  Molecular approaches to improve the insecticidal activity of Bacillus thuringiensis Cry toxins.

Authors:  Wagner A Lucena; Patrícia B Pelegrini; Diogo Martins-de-Sa; Fernando C A Fonseca; Jose E Gomes; Leonardo L P de Macedo; Maria Cristina M da Silva; Raquel S Oliveira; Maria F Grossi-de-Sa
Journal:  Toxins (Basel)       Date:  2014-08-13       Impact factor: 4.546

Review 3.  Making 3D-Cry Toxin Mutants: Much More Than a Tool of Understanding Toxins Mechanism of Action.

Authors:  Susana Vílchez
Journal:  Toxins (Basel)       Date:  2020-09-16       Impact factor: 4.546

4.  Chronic Responses of Daphnia magna Under Dietary Exposure to Leaves of a Transgenic (Event MON810) Bt-Maize Hybrid and its Conventional Near-Isoline.

Authors:  Daniel Ferreira Holderbaum; Marek Cuhra; Fern Wickson; Afonso Inácio Orth; Rubens Onofre Nodari; Thomas Bøhn
Journal:  J Toxicol Environ Health A       Date:  2015

5.  A transgenic approach for controlling Lygus in cotton.

Authors:  Anilkumar Gowda; Timothy J Rydel; Andrew M Wollacott; Robert S Brown; Waseem Akbar; Thomas L Clark; Stanislaw Flasinski; Jeffrey R Nageotte; Andrew C Read; Xiaohong Shi; Brent J Werner; Michael J Pleau; James A Baum
Journal:  Nat Commun       Date:  2016-07-18       Impact factor: 14.919
  5 in total

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