Fernanda Brasil Baboni1, Odilon Guariza Filho2, Andréa Novais Moreno3, Edvaldo Antonio Ribeiro Rosa4. 1. Postgraduate student, Graduate Program in Dentistry-Orthodontics, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil. 2. Associate professor, Graduate Program in Dentistry-Orthodontics, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil. 3. Associate professor, Graduate Program in Health Sciences, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil. 4. Professor, Graduate Program in Dentistry-Oral Biosciences, Pontifical Catholic University of Paraná, Curitiba, Paraná, Brazil. Electronic address: edvaldo.rosa@pucpr.br.
Abstract
INTRODUCTION: An experimental analysis was made to quantify the adherence rates and the biofilm formation capacity of Streptococcus mutans ATCC25175 and Candida albicans SC5314 on orthodontic material surfaces in the presence of cigarette smoke condensate (CSC). METHODS: Metal brackets, bands, acrylic resin, and polyurethane elastic rings were coated with stimulated saliva and submitted to adhesion and biofilm formation tests with and without CSC in a dynamic system. RESULTS: The CSC increased the adhesion of S mutans ATCC25175 to the acquired pellicle (P <0.05) for bands (4.08 times), acrylic resin (2.89 times), and brackets (3.37 times) and reduced it in polyurethane elastic (2.66 times; P <0.05). S mutans ATCC25175 biofilm biomass was increased by CSC only on brackets (1.60 times; P <0.05). In the presence of CSC, the adhesion of C albicans SC5314 increased (P <0.05) on bands (1.81 times), brackets (9.61 times), elastics (29,133 times), and acrylic resin (177 times). Greater formation of C albicans SC5314 biofilm caused by CSC (P <0.05) was observed on acrylic resin (2.13 times) and brackets (2.32 times). CONCLUSIONS: The results indicated that cigarette tobacco smoke can interfere with the adhesion and biofilm formation of these microorganisms to various orthodontic materials.
INTRODUCTION: An experimental analysis was made to quantify the adherence rates and the biofilm formation capacity of Streptococcus mutans ATCC25175 and Candida albicans SC5314 on orthodontic material surfaces in the presence of cigarette smoke condensate (CSC). METHODS: Metal brackets, bands, acrylic resin, and polyurethane elastic rings were coated with stimulated saliva and submitted to adhesion and biofilm formation tests with and without CSC in a dynamic system. RESULTS: The CSC increased the adhesion of S mutans ATCC25175 to the acquired pellicle (P <0.05) for bands (4.08 times), acrylic resin (2.89 times), and brackets (3.37 times) and reduced it in polyurethane elastic (2.66 times; P <0.05). S mutans ATCC25175 biofilm biomass was increased by CSC only on brackets (1.60 times; P <0.05). In the presence of CSC, the adhesion of C albicans SC5314 increased (P <0.05) on bands (1.81 times), brackets (9.61 times), elastics (29,133 times), and acrylic resin (177 times). Greater formation of C albicans SC5314 biofilm caused by CSC (P <0.05) was observed on acrylic resin (2.13 times) and brackets (2.32 times). CONCLUSIONS: The results indicated that cigarette tobacco smoke can interfere with the adhesion and biofilm formation of these microorganisms to various orthodontic materials.
Authors: Kinga Grzegocka; Paweł Krzyściak; Anna Hille-Padalis; Jolanta E Loster; Katarzyna Talaga-Ćwiertnia; Bartłomiej W Loster Journal: BMC Oral Health Date: 2020-10-10 Impact factor: 2.757