BACKGROUND: MG7-Ag is a kind of gastric cancer-specific tumor-associated antigen and has been investigated to serve as a marker of gastric cancer for early diagnosis. METHODS: Surface plasmon resonance (SPR) sensor was used for the detection of MG7-Ag in the sera of gastric cancer patients to develop an innovative, simple and rapid assay method for early diagnosis. The specific monoclonal MG7 antibodies were used as capture and detection receptors which were immobilized on the surface of SPR sensor chips for MG7-Ag identification in the human sera. The measurements include 9 cases of gastric cancer patients and 2 cases of healthy blood donors and a MKN45 cancer cell lysate solution sample for positive control. RESULTS: The binding of MG7-Ag onto the sensor surface was observed from SPR spectra. The sera of most gastric cancer patients revealed much higher expression level of MG7-Ag than healthy human sera did in SPR measurement. CONCLUSION: The initial results demonstrate that the SPR biosensor has the potential for its application in the early diagnosis of gastric cancer. However, more tests need to be done to confirm the detection limitation and the criterion for cancer risk evaluation in early diagnosis.
BACKGROUND:MG7-Ag is a kind of gastric cancer-specific tumor-associated antigen and has been investigated to serve as a marker of gastric cancer for early diagnosis. METHODS: Surface plasmon resonance (SPR) sensor was used for the detection of MG7-Ag in the sera of gastric cancerpatients to develop an innovative, simple and rapid assay method for early diagnosis. The specific monoclonal MG7 antibodies were used as capture and detection receptors which were immobilized on the surface of SPR sensor chips for MG7-Ag identification in the human sera. The measurements include 9 cases of gastric cancerpatients and 2 cases of healthy blood donors and a MKN45 cancer cell lysate solution sample for positive control. RESULTS: The binding of MG7-Ag onto the sensor surface was observed from SPR spectra. The sera of most gastric cancerpatients revealed much higher expression level of MG7-Ag than healthy human sera did in SPR measurement. CONCLUSION: The initial results demonstrate that the SPR biosensor has the potential for its application in the early diagnosis of gastric cancer. However, more tests need to be done to confirm the detection limitation and the criterion for cancer risk evaluation in early diagnosis.
Authors: Victor Hugo Perez-Gonzalez; Roberto Carlos Gallo-Villanueva; Sergio Camacho-Leon; Jose Isabel Gomez-Quiñones; Jose Manuel Rodriguez-Delgado; Sergio Omar Martinez-Chapa Journal: IET Nanobiotechnol Date: 2016-10 Impact factor: 1.847