PURPOSE: To investigate the utility of noninvasive magnetic resonance imaging (MRI) protocols to demonstrate pathological differences between rats and mice after spinal cord injury (SCI). Rats and mice are commonly used to model SCI; however, histology and immunohistochemistry have shown differences in neuropathology between the two species, including cavity formation and scar/inflammatory responses. MATERIALS AND METHODS: Moderate contusion SCI was performed on adult male rats and mice. At 28 days postinjury, animals underwent T1-weighted (T1W), with or without gadolinium contrast, or T2-weighted (T2W) magnetic resonance imaging (MRI), to be compared with histology at the same timepoint. RESULTS: In both species, all MRI methods demonstrated changes in spinal cord anatomy. Immunohistochemistry indicated that T2W accurately reflected areas of inflammation and glial scar formation in rats and mice. Quantitation of lesion volume by histology and functional performance correlated best with T2W measurements in both species. Gadolinium contrast accurately reflected the blood-spinal cord-barrier permeability in both species, which appeared greater in rats than in mice. CONCLUSION: These data demonstrate that MRI, with either a T1W or T2W protocol, can effectively distinguish pathological differences between rats and mice.
PURPOSE: To investigate the utility of noninvasive magnetic resonance imaging (MRI) protocols to demonstrate pathological differences between rats and mice after spinal cord injury (SCI). Rats and mice are commonly used to model SCI; however, histology and immunohistochemistry have shown differences in neuropathology between the two species, including cavity formation and scar/inflammatory responses. MATERIALS AND METHODS: Moderate contusion SCI was performed on adult male rats and mice. At 28 days postinjury, animals underwent T1-weighted (T1W), with or without gadolinium contrast, or T2-weighted (T2W) magnetic resonance imaging (MRI), to be compared with histology at the same timepoint. RESULTS: In both species, all MRI methods demonstrated changes in spinal cord anatomy. Immunohistochemistry indicated that T2W accurately reflected areas of inflammation and glial scar formation in rats and mice. Quantitation of lesion volume by histology and functional performance correlated best with T2W measurements in both species. Gadolinium contrast accurately reflected the blood-spinal cord-barrier permeability in both species, which appeared greater in rats than in mice. CONCLUSION: These data demonstrate that MRI, with either a T1W or T2W protocol, can effectively distinguish pathological differences between rats and mice.
Authors: M A Marciello; A E Flanders; G J Herbison; D M Schaefer; D P Friedman; J I Lane Journal: Arch Phys Med Rehabil Date: 1993-09 Impact factor: 3.966
Authors: S D Weirich; H B Cotler; P A Narayana; J D Hazle; E F Jackson; K J Coupe; C L McDonald; L A Langford; J H Harris Journal: Spine (Phila Pa 1976) Date: 1990-07 Impact factor: 3.468
Authors: William D Whetstone; Jung-Yu C Hsu; Manuel Eisenberg; Zena Werb; Linda J Noble-Haeusslein Journal: J Neurosci Res Date: 2003-10-15 Impact factor: 4.164
Authors: Tsen-Hsuan Lin; Peng Sun; Mitchell Hallman; Fay C Hwang; Michael Wallendorf; Wilson Z Ray; William M Spees; Sheng-Kwei Song Journal: J Neurotrauma Date: 2019-01-11 Impact factor: 5.269
Authors: Ramona E von Leden; Reed G Selwyn; Shalini Jaiswal; Colin M Wilson; Guzal Khayrullina; Kimberly R Byrnes Journal: Neurosci Lett Date: 2016-04-12 Impact factor: 3.046
Authors: Brian J Kelley; Noam Y Harel; Chang-Yeon Kim; Xenophon Papademetris; Daniel Coman; Xingxing Wang; Omar Hasan; Adam Kaufman; Ronen Globinsky; Lawrence H Staib; William B J Cafferty; Fahmeed Hyder; Stephen M Strittmatter Journal: J Neurotrauma Date: 2014-07-08 Impact factor: 5.269
Authors: Richard I Tuxworth; Matthew J Taylor; Ane Martin Anduaga; Alaa Hussien-Ali; Sotiroula Chatzimatthaiou; Joanne Longland; Adam M Thompson; Sharif Almutiri; Pavlos Alifragis; Charalambos P Kyriacou; Boris Kysela; Zubair Ahmed Journal: Brain Commun Date: 2019-07-02